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| Status |
Public on Sep 05, 2017 |
| Title |
Vitamin D regulates expression of multiple genes including CD14 and CD180 to modulate the syncytiotrophoblast response to lipopolysaccharide |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We assessed the response of primary cultures of human trophoblasts to bioactive 1,25 (OH)2D3 and used whole genome microarray to identify innovative hypotheses.
Data indicate that bioactive vitamin D has wide-spread effects on villous gene expression in general, and a specific affect effect on the immune response.
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| Overall design |
Primary human villous cytotrophoblasts were isolated as described {Chen et al., 2010, Am J Physiol Cell Physiol, 299, C968-76; Chen et al., 2015, Placenta, 36, 847-53} and washed thoroughly 4 h after plating, to eliminate villous fragments and nonviable cells.
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| Contributor(s) |
Longtine MS, Cvitic S, Colvin BN, Chen B, Desoye G, Nelson MD |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 28, 2017 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Silvija Tokic |
| E-mail(s) |
silvija.tokic@medunigraz.at
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| Organization name |
Medical University of Graz
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| Street address |
Auenbruggerplatz 14
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| City |
Graz |
| ZIP/Postal code |
8036 |
| Country |
Austria |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA377274 |
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