|
| Status |
Public on Dec 04, 2007 |
| Title |
Erb rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
infected with recombinant adenovirus bearing cDNA for ERbeta; rep1
|
| Organism |
Homo sapiens |
| Characteristics |
MDA-MB-231
|
| Treatment protocol |
Infected cells were treated with 17beta-estradiol for 6h
|
| Growth protocol |
MDA-MB-231 cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or ERbeta mutant defective in binding to ERE (ERbDBD) in medium without phenol red and containing 10% CD-FBS for 48h. Cells were then treated with 1 nM estradiol-17beta for 6h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected by trpsinization, pelleted. Total RNA was extracted using Qiagen mini RNEasy kit.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled cDNA was prepared from 20 ng total RNA using the NuGen Ovation kit per instructions provided with the kit.
|
| |
|
| Hybridization protocol |
2 micrograms biotin-labeled, fragmented, single-stranded cDNA were hybridized with each array using the standard Affymetrix protocol with an Affymetrix hybridization oven and Fluidics Station 450.
|
| Scan protocol |
After washing and staining with phycoerythrin-streptavidin per the standard Affymetrix protocol, arrays were scanned with an Affymetrix Scanner 3000
|
| Description |
Erb_rep1
|
| Data processing |
Cel files were used to perform GCRMA normalization implemented by the Bioconductor gcrma package. Detection calls and detection P values were computed with Gene Chip Operating Software (Affymetrix)
|
| |
|
| Submission date |
Dec 03, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Stephen Welle |
| E-mail(s) |
swelle@rochester.rr.com
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE9759 |
Response to estradiol-ERbeta and estradiol-ERbeta ERE binding defective mutant |
| GSE9761 |
Response to estradiol-ERalpha, estradiol-Erbeta, and ERE Binding defective mutants |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
