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| Status |
Public on Dec 04, 2007 |
| Title |
Response to estradiol-ERbeta and estradiol-ERbeta ERE binding defective mutant |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
In addition to the estrogen responsive element (ERE)-dependent gene expression, E2-ERbeta regulates transcription through functional interactions with transfactors bound to their cognate regulatory elements on DNA, hence the ERE-independent signaling pathway. However, the relative importance of the ERE-independent pathway in E2-ERbeta signaling is unclear. Our studies in infected ER-negative cell models with an ERbeta mutant (ERbetaDBD) that functions exclusively at the ERE-independent pathway demonstrated that genomic responses assessed by microarrays from the ERE-independent pathway to E2-ERbeta are not sufficient to alter cellular growth, death or motility. These findings suggest that the ERE-dependent pathway is the canonical E2-ERbeta signaling in model cell lines.
Keywords: MDA-MB-231 cells
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| Overall design |
Cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or a cDNA for ERbeta, or cDNA for ERbeta mutant defective in binding to ERE (ERbDBD). for 48 hours. Infected cells were then treated with 1 nM Estradiol 17beta for 6h. All experiments were repeated six independent times conducted at at six different days
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| Contributor(s) |
Muyan M, Li X, Nott SL, Huang Y, Hilf R, Bambara RA, Qiu X, Yakovlev A, Welle S |
| Citation(s) |
19321454 |
| Submission date |
Dec 03, 2007 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Stephen Welle |
| E-mail(s) |
swelle@rochester.rr.com
|
| Organization name |
University of Rochester
|
| Street address |
601 Elmwood Avenue
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (18)
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| This SubSeries is part of SuperSeries: |
| GSE9761 |
Response to estradiol-ERalpha, estradiol-Erbeta, and ERE Binding defective mutants |
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| Relations |
| BioProject |
PRJNA105109 |
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