|
Status |
Public on Jan 08, 2019 |
Title |
Shah_026 Nik+/+ |
Sample type |
RNA |
|
|
Source name |
Normal Colon
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: Nik+/+ colon phenotype: normal age: 3 months old
|
Treatment protocol |
Normal and inflamed colon tissues were got from 3 months old mice
|
Growth protocol |
All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol from 400 ng total RNA (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2).
|
|
|
Hybridization protocol |
Following fragmentation, 3.7 ug of cDNA were hybridized at 48C on a Mouse Gene 2.1 ST Array strip using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system.
|
Description |
Gene expression data from colons.
|
Data processing |
Data was analyzed using the oligo Bioconductor package implemented in the R-statistical environment version 3.0.0. RMA was used to fit log2 transformed expression values.
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|
|
Submission date |
Aug 29, 2016 |
Last update date |
Jan 09, 2019 |
Contact name |
XIANG XUE |
E-mail(s) |
xxue@umich.edu
|
Phone |
7346155041
|
Organization name |
Umich
|
Street address |
1301 E. Catherine Street
|
City |
Ann Arbor |
State/province |
Michigan |
ZIP/Postal code |
48109 |
Country |
USA |
|
|
Platform ID |
GPL17400 |
Series (1) |
GSE86194 |
Gene Expression profiles of normal and inflamed colon tissues from Nik+/+, NikΔIE |
|