|
| Status |
Public on Jan 08, 2019 |
| Title |
Shah_026 Nik+/+ |
| Sample type |
RNA |
| |
|
| Source name |
Normal Colon
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: Nik+/+
colon phenotype: normal
age: 3 months old
|
| Treatment protocol |
Normal and inflamed colon tissues were got from 3 months old mice
|
| Growth protocol |
All mice were C57BL/6 background and maintained in standard cages in a light and temperature-controlled room and were allowed standard chow and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol from 400 ng total RNA (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2).
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.7 ug of cDNA were hybridized at 48C on a Mouse Gene 2.1 ST Array strip using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system.
|
| Description |
Gene expression data from colons.
|
| Data processing |
Data was analyzed using the oligo Bioconductor package implemented in the R-statistical environment version 3.0.0. RMA was used to fit log2 transformed expression values.
|
| |
|
| Submission date |
Aug 29, 2016 |
| Last update date |
Jan 09, 2019 |
| Contact name |
XIANG XUE |
| E-mail(s) |
xxue@umich.edu
|
| Phone |
7346155041
|
| Organization name |
Umich
|
| Street address |
1301 E. Catherine Street
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE86194 |
Gene Expression profiles of normal and inflamed colon tissues from Nik+/+, NikΔIE |
|
