|
| Status |
Public on Jun 01, 2018 |
| Title |
291 Tumor |
| Sample type |
RNA |
| |
|
| Source name |
Tumor 291
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Tumor from GEMM
|
| Treatment protocol |
Tumors were generated by treatment of mice with doxycycline. Control mice were also treated with doxycycline before fallopian tube extraction.
|
| Growth protocol |
Mice were bred following the IACUC protocol at MD Anderson Cancer Center mouse facility.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix U133 Plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from tumors of doxycycline inducible GEMM
|
| Data processing |
Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
|
| |
|
| Submission date |
Jun 06, 2016 |
| Last update date |
Jun 01, 2018 |
| Contact name |
Sharmistha Sarkar |
| Organization name |
The University of Texas MD Anderson Cancer Center
|
| Department |
Genomic Medicine
|
| Street address |
1515 Holcombe Boulevard
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL16368 |
| Series (1) |
| GSE82319 |
PRKCI promotes immune suppression in ovarian cancer through YAP1 |
|
