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| Status |
Public on Jun 01, 2018 |
| Title |
PRKCI promotes immune suppression in ovarian cancer through YAP1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
A key feature of high-grade serous ovarian carcinoma (HGSOC) is frequent amplification of the 3q26 locus harboring PRKC-iota (PRKCI). Here, we show that PRKCI is also expressed in early fallopian tube lesions, called Serous Tubal Intraepithelial Carcinoma. Transgenic mouse studies establish PRKCI as an ovarian cancer specific oncogene and system level and functional analyses identify YAP1 as a downstream effector in tumor progression. Mechanistically, the oncogenic activity of the PRKCI-YAP1 axis relates in part to the upregulation of TNFα to promote an immune suppressive tumor microenvironment characterized by an abundance of myeloid-derived suppressor cells and inhibition of cytotoxic T cell infiltration. In human ovarian cancers, high PRKCI expression also correlates with high expression of YAP1 and low infiltration of cytotoxic T-cell. The PRKCI-YAP1 regulation of the tumor immunity provides a therapeutic strategy for highly lethal ovarian cancer.
Robust multi-array average (RMA) method was used with default options (with background correction, quantile normalization, and log transformation) to normalize raw data from batches using R/Bioconductor‘s affy package
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| Overall design |
Charterization of transcriptional alterations of tumors from a genetically engineered mouse model (GEMM) compared with fallopian tube of wild type mice.
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| Contributor(s) |
Sarkar S, Bristow CA, Chin L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 06, 2016 |
| Last update date |
Jun 02, 2018 |
| Contact name |
Sharmistha Sarkar |
| Organization name |
The University of Texas MD Anderson Cancer Center
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| Department |
Genomic Medicine
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| Street address |
1515 Holcombe Boulevard
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL16368 |
[Mouse4302_Mm_ENTREZG] Affymetrix GeneChip Mouse Genome 430 2.0 Array [Brainarray Version 16] |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA324608 |
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