|
| Status |
Public on Dec 12, 2015 |
| Title |
H1299 siCBP 48h #2 |
| Sample type |
RNA |
| |
|
| Source name |
H1299 siCBP 48h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
cell line: H1299
|
| Treatment protocol |
CBP and p300 wild-type H1299 cancer cells, CBP-KO H1299 cells, and p300-KO H1299 cells were transfected with siRNAs (siNT, sip300 D1, siCBP D2) using the Lipofectamine RNAiMAX reagent. After 48 h, total RNA was extracted.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the Qiagen RNeasy kit.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|
| |
|
| Hybridization protocol |
cDNA was hybridized to Agilent microarrays (SurePrint G3 Human Gene Expression 8 x 60K Ver.1.0, G4851: 42405 probes) using a Gene Expression Hybridization Kit (Agilent Technologies) for 16 h at 65°C in duplicate.
|
| Scan protocol |
After the arrays were washed using the Gene Expression Wash Pack (Agilent Technologies), the data were extracted using an Agilent scanner.
|
| Description |
Gene expression 48 h after siRNA knockdown
|
| Data processing |
The arrays were first analyzed using the Feature Extraction software (Agilent Technologies).
|
| |
|
| Submission date |
Oct 02, 2015 |
| Last update date |
Dec 12, 2015 |
| Contact name |
Takashi Kohno |
| E-mail(s) |
tkkohno@ncc.go.jp
|
| Phone |
+81-3-3547-2511 (4650)
|
| Organization name |
National Cancer Center Research Institute
|
| Lab |
Division of Genome Biology
|
| Street address |
1-1, Tsukiji 5-chome
|
| City |
Chuo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE73682 |
Development of gene expression signatures for knockdown of synthetic lethal gene |
|
