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Sample GSM1900746 Query DataSets for GSM1900746
Status Public on Dec 12, 2015
Title H1299 siCBP 48h #2
Sample type RNA
Source name H1299 siCBP 48h
Organism Homo sapiens
Characteristics tissue: Lung
cell line: H1299
Treatment protocol CBP and p300 wild-type H1299 cancer cells, CBP-KO H1299 cells, and p300-KO H1299 cells were transfected with siRNAs (siNT, sip300 D1, siCBP D2) using the Lipofectamine RNAiMAX reagent. After 48 h, total RNA was extracted.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Qiagen RNeasy kit.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.5 ug RNA using the One-Color Low RNA Input Linear Amplification PLUS kit (Agilent) according to the manufacturer's instructions, followed by RNAeasy column purification (QIAGEN, Valencia, CA). Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol cDNA was hybridized to Agilent microarrays (SurePrint G3 Human Gene Expression 8 x 60K Ver.1.0, G4851: 42405 probes) using a Gene Expression Hybridization Kit (Agilent Technologies) for 16 h at 65°C in duplicate.
Scan protocol After the arrays were washed using the Gene Expression Wash Pack (Agilent Technologies), the data were extracted using an Agilent scanner.
Description Gene expression 48 h after siRNA knockdown
Data processing The arrays were first analyzed using the Feature Extraction software (Agilent Technologies).
Submission date Oct 02, 2015
Last update date Dec 12, 2015
Contact name Takashi Kohno
Phone +81-3-3547-2511 (4650)
Organization name National Cancer Center Research Institute
Lab Division of Genome Biology
Street address 1-1, Tsukiji 5-chome
City Chuo-ku
State/province Tokyo
ZIP/Postal code 104-0045
Country Japan
Platform ID GPL13607
Series (1)
GSE73682 Development of gene expression signatures for knockdown of synthetic lethal gene

Data table header descriptions
VALUE Normalized signal intensity

Data table
1 5.32E+04
2 1.09E+01
3 4.46E+00
4 8.14E+02
5 8.54E+02
6 6.07E+01
7 5.26E+03
8 2.22E+03
9 1.27E+01
10 7.40E+01
11 4.72E+00
12 5.51E+02
13 2.77E+02
14 9.69E+02
15 1.67E+04
16 4.81E+00
17 2.20E+02
18 8.18E+01
19 5.66E+00
20 1.04E+03

Total number of rows: 62976

Table truncated, full table size 911 Kbytes.

Supplementary file Size Download File type/resource
GSM1900746_US85000263_252800421753_S01_GE1_107_Sep09_2_3.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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