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Sample GSM1892502 Query DataSets for GSM1892502
Status Public on Dec 31, 2018
Title BAL_SARC_21
Sample type RNA
Source name SARC_BAL cells
Organism Homo sapiens
Characteristics diagnosis: SARC
cohort: Freiburg
age (yrs): 39
gender (male=1): 1
cell type: bronchoalveolar lavage (BAL) cells
Extracted molecule total RNA
Extraction protocol Isolation protocol: BAL cells were obtained by bronchoscopy, immediately isolated and 1 ml of TRIzol was added to the cell pellet, which was then immediately snap frozen in -80 celsius.
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA
Hybridization protocol Following fragmentation, cRNA were hybridized on Affymetrix Whole-Transcript Human Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using a CGS30007G scanner (serialnumber 54713600)
Description HUGENE-1_0-ST-V1 SARC_21
Data processing Raw CEL files were RMA normalized using the R/Bioconductor package oligo (V.1.32.0) with default settings. Gene expression data was normalized to the geometric mean of all samples using quantile normalization and expression levels were summarized.
Submission date Sep 24, 2015
Last update date Dec 31, 2018
Contact name Antje Prasse
Organization name Medical School Hannover
Department Pneumology
Lab Prasse OE6870
Street address Carl-Neubergstrasse 1
City Hannover
ZIP/Postal code 30625
Country Germany
Platform ID GPL10739
Series (2)
GSE70867 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells
GSE73394 BAL cell gene expression is predictive of Mortality in Idiopathic Pulmonary Fibrosis and enriched for Genes of Airway Basal Cells (III)

Data table header descriptions
VALUE RMA singal intensity

Data table
7892501 4.616602951
7892502 5.779967184
7892503 5.319356758
7892504 10.02449186
7892505 5.048620191
7892506 6.070044177
7892507 5.883919267
7892508 6.59120181
7892509 11.46423992
7892510 5.853588486
7892511 5.212243254
7892512 7.257078405
7892513 5.1930099
7892514 11.73744022
7892515 10.18569775
7892516 4.887396022
7892517 6.80898195
7892518 3.842259983
7892519 5.236630856
7892520 9.837841228

Total number of rows: 257430

Table truncated, full table size 5001 Kbytes.

Supplementary file Size Download File type/resource
GSM1892502_HUGENE-1_0-ST-V1_SARC_21.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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