|
| Status |
Public on Jun 01, 2021 |
| Title |
MeWo_Scr-shRNA |
| Sample type |
RNA |
| |
|
| Source name |
Scr knockdown MeWo cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MeWo
cell type: melanoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
|
| Label |
Cy3
|
| Label protocol |
cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human Gene Expression Microarray 8x60K v2; Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version 9.5.1.1 (Agilent Technologies).
|
| Description |
Gene expression in Scr knockdown MeWo cells
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5.1.1 (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. In addition, the raw signal intensities of two samples were log2-transformed and normalized by the quantile algorithm with the Bioconductor.
|
| |
|
| Submission date |
May 28, 2015 |
| Last update date |
Jun 01, 2021 |
| Contact name |
Yuhui Huang |
| E-mail(s) |
xiaoya55221@yahoo.co.jp
|
| Organization name |
Faculty of Agriculture, Kyushu University
|
| Department |
Department of Bioscience and Biotechnology
|
| Lab |
Food science and technology
|
| Street address |
Hakozaki 6-10-1
|
| City |
Fukuoka |
| ZIP/Postal code |
812-8581 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE69328 |
The effect of STARD3 knockdown on melanoma cell line MeWo |
|
