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Sample GSM1697875 Query DataSets for GSM1697875
Status Public on Jun 01, 2021
Title MeWo_Scr-shRNA
Sample type RNA
Source name Scr knockdown MeWo cells
Organism Homo sapiens
Characteristics cell line: MeWo
cell type: melanoma
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from cells using TRIzol Reagent (nitrogen) and purified using SV Total RNA Isolation System (Promega)
Label Cy3
Label protocol cRNA was amplified and labelled using a Low input Quick Amp Labelling Kit (Agilent Technologies).
Hybridization protocol cRNA was hybridized to a 60K 60-mer oligomicroarray (SurePrint G3 Human Gene Expression Microarray 8x60K v2; Agilent Technologies) according to the manufacturer's instructions.
Scan protocol The hybridized microarray slides were scanned using an Agilent scanner. The relative hybridization intensities and background hybridization values were calculated using Feature Extraction Software version (Agilent Technologies).
Description Gene expression in Scr knockdown MeWo cells
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent) using default parameters to obtain background subtracted and spatially detrended Processed Signal intensities. The raw signal intensities and flags for each probe were calculated from the hybridization intensities and spot information according to the procedures recommended by Agilent Technologies using the Flag criteria in the GeneSpring Software. In addition, the raw signal intensities of two samples were log2-transformed and normalized by the quantile algorithm with the Bioconductor.
Submission date May 28, 2015
Last update date Jun 01, 2021
Contact name Yuhui Huang
Organization name Faculty of Agriculture, Kyushu University
Department Department of Bioscience and Biotechnology
Lab Food science and technology
Street address Hakozaki 6-10-1
City Fukuoka
ZIP/Postal code 812-8581
Country Japan
Platform ID GPL17077
Series (1)
GSE69328 The effect of STARD3 knockdown on melanoma cell line MeWo

Data table header descriptions
VALUE quantile normalized signal, non-log scaled

Data table
A_19_P00315452 514.75845 P
A_19_P00315459 147.929 P
A_19_P00315482 7.808965 A
A_19_P00315492 20.02117 P
A_19_P00315493 50.52221 P
A_19_P00315502 3.592478 A
A_19_P00315506 19.35341 P
A_19_P00315518 2.9635745 A
A_19_P00315519 2.8649505 A
A_19_P00315524 14.34109 P
A_19_P00315528 10.297477 A
A_19_P00315529 4.418534 A
A_19_P00315538 604.6366 P
A_19_P00315541 5.006211 A
A_19_P00315543 13.608775 A
A_19_P00315550 129.19015 P
A_19_P00315551 150.4905 P
A_19_P00315554 651.59335 P
A_19_P00315581 2481.613 P
A_19_P00315583 44.056665 P

Total number of rows: 50599

Table truncated, full table size 1240 Kbytes.

Supplementary file Size Download File type/resource
GSM1697875_US11030397_253949424944_S01_GE1_107_Sep09_1_3.txt.gz 12.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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