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Sample GSM1104066 Query DataSets for GSM1104066
Status Public on May 27, 2013
Title proB-cell line_Runx1-ERt2_EtOH
Sample type RNA
Source name cell line BMiFLT3(15-3)
Organism Mus musculus
Characteristics cell line: BMiFLT3(15-3)
cell type: proB cells from a murine BCP-ALL
genetic modification: cell transduced with Runx1-ERt2
treatment: control
Treatment protocol 24 hours before sorting the cells were either treated with 0.2 µM OHT to ensure Runx1-ERt2 translocation into the nucleus or treated with the appropriate amount of ethanol as a control. For FACS-sort cells were stained with an antibody against B220. B220 positive cells were isolated and cell pellets were frozen at -80 °C until further processing steps were performed.
Growth protocol Cells were routinely grown in α-MEM with 10% FCS, glutamine and sodium pyruvate.
Extracted molecule total RNA
Extraction protocol RNA was extracted (NucleoSpin® RNA II; Agilent Technologies), according to the manufacturer's instructions by Miltenyi Biotec.
Label Cy3
Label protocol Cy3-labeled cRNA was prepared using the Agilent Low Input Quick Amp Labeling Kit (Agilent Technologies) following manufacturer´s protocol. The amount of cRNA and the dye-incorporation rate were checked by using the ND-1000 Spectrophotometer (NanoDrop Technologies) by Miltenyi Biotec.
Hybridization protocol Hybridization was peformed according to manufacturer´s protocol using the Agilent Gene Expression Hybridization Kit (Agilent Technologies). 1.65 µg Cy3-labeled fragmented cRNA was hybridized overnight (17 hrs., 65 °C) to Agilent Whole Mouse Genome Oligo Microarrays 4x44K by Miltenyi Biotec.
Scan protocol Fluorescence signals were scanned using the Agilent´s Microarray Scanner System (Agilent Technologies). The Agilent Feature Extraction Software (EFS) was used to read out and process the microarray image files.
Data processing The images were analyzed using the Agilent G2567AA Feature Extraction Software v.9.1.
Submission date Mar 22, 2013
Last update date May 28, 2013
Contact name Neele Margarete Kriebitzsch
Organization name Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
Lab Molecular Pathology
Street address Martinistraße 52
City Hamburg
ZIP/Postal code 20251
Country Germany
Platform ID GPL11202
Series (2)
GSE45424 Gene expression analysis to identify Runx1 target genes in B-cell progenitors
GSE45425 Runx1 targets in early B-cell progenitors

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_51_P100034 58.912712
A_51_P100174 79.144368
A_51_P100208 0.130476
A_51_P100289 49.415219
A_51_P100298 0.115944
A_51_P100309 0.10726
A_51_P100327 30.412499
A_51_P100347 2.10515
A_51_P100519 0.111384
A_51_P100537 0.121987
A_51_P100573 13.501134
A_51_P100624 0.108366
A_51_P100625 1.507926
A_51_P100768 1.17138
A_51_P100776 3.730117
A_51_P100787 110.832067
A_51_P100828 106.183209
A_51_P100852 5.206154
A_51_P100991 0.516116
A_51_P100997 6.759181

Total number of rows: 39429

Table truncated, full table size 884 Kbytes.

Supplementary file Size Download File type/resource
GSM1104066_252665512013_S01_GE1_105_Jan09_1_1.txt.gz 8.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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