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| Status |
Public on May 27, 2013 |
| Title |
Gene expression analysis to identify Runx1 target genes in B-cell progenitors |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The t(12;21) chromosomal translocation, targeting the gene encoding the RUNX1 transcription factor, is observed in 25% of pediatric acute lymphoblastic leukemia (ALL) and is an initiating event in the disease. To elucidate the mechanism by which RUNX1 disruption initiates leukemogenesis, we investigated its normal role in murine B-cell development. Gene expression analysis and genome-wide Runx1-occupancy studies support the hypothesis that Runx1 reinforces the transcription factor network in B-cell progenitors governing early B-cell survival and development .
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| Overall design |
1) To identify Runx1 target genes in early B-cell progenitors, we used a whole genome microarray for analyzing the gene expression profile in a proB-cell line [BMiFLT3(15-3)] engineered to overexpresses the Runx1 transcription factor fused to the ligand binding domain of the estrogen receptor. The use of an inducible Runx1 fusion protein allowed specific translocation of Runx1 into the nucleus activation of Runx1 target genes.
2) To identify Runx1 target genes in early B-cell progenitors, we used a whole genome microarray for analyzing the gene expression profiles of proB-cells (CD19+/B220med/CD93+) isolated from Runx1+/+Cd79ahCre/+ and Runx1fl/flCd79ahCre/+ mice. Two independent experiments were peformed.
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| Contributor(s) |
Niebuhr B, Kriebitzsch N, Stocking C |
| Citation(s) |
23704093 |
| Submission date |
Mar 22, 2013 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Neele Margarete Kriebitzsch |
| E-mail(s) |
neele.kriebitzsch@hpi.uni-hamburg.de
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| Organization name |
Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
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| Lab |
Molecular Pathology
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| Street address |
Martinistraße 52
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| City |
Hamburg |
| ZIP/Postal code |
20251 |
| Country |
Germany |
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| Platforms (2) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE45425 |
Runx1 targets in early B-cell progenitors |
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| Relations |
| BioProject |
PRJNA193698 |
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