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Series GSE45424 Query DataSets for GSE45424
Status Public on May 27, 2013
Title Gene expression analysis to identify Runx1 target genes in B-cell progenitors
Organism Mus musculus
Experiment type Expression profiling by array
Summary The t(12;21) chromosomal translocation, targeting the gene encoding the RUNX1 transcription factor, is observed in 25% of pediatric acute lymphoblastic leukemia (ALL) and is an initiating event in the disease. To elucidate the mechanism by which RUNX1 disruption initiates leukemogenesis, we investigated its normal role in murine B-cell development. Gene expression analysis and genome-wide Runx1-occupancy studies support the hypothesis that Runx1 reinforces the transcription factor network in B-cell progenitors governing early B-cell survival and development .
 
Overall design 1) To identify Runx1 target genes in early B-cell progenitors, we used a whole genome microarray for analyzing the gene expression profile in a proB-cell line [BMiFLT3(15-3)] engineered to overexpresses the Runx1 transcription factor fused to the ligand binding domain of the estrogen receptor. The use of an inducible Runx1 fusion protein allowed specific translocation of Runx1 into the nucleus activation of Runx1 target genes.
2) To identify Runx1 target genes in early B-cell progenitors, we used a whole genome microarray for analyzing the gene expression profiles of proB-cells (CD19+/B220med/CD93+) isolated from Runx1+/+Cd79ahCre/+ and Runx1fl/flCd79ahCre/+ mice. Two independent experiments were peformed.
 
Contributor(s) Niebuhr B, Kriebitzsch N, Stocking C
Citation(s) 23704093
Submission date Mar 22, 2013
Last update date Nov 01, 2017
Contact name Neele Margarete Kriebitzsch
E-mail(s) neele.kriebitzsch@hpi.uni-hamburg.de
Organization name Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology
Lab Molecular Pathology
Street address Martinistra├če 52
City Hamburg
ZIP/Postal code 20251
Country Germany
 
Platforms (2)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (8)
GSM1104066 proB-cell line_Runx1-ERt2_EtOH
GSM1104067 proB-cell line_Runx1-ERt2_OHT
GSM1104068 proB-cell line_ERt2_EtOH
This SubSeries is part of SuperSeries:
GSE45425 Runx1 targets in early B-cell progenitors
Relations
BioProject PRJNA193698

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE45424_RAW.tar 71.6 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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