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Sample GSM1028104 Query DataSets for GSM1028104
Status Public on Feb 01, 2013
Title Reh cells_control_12h
Sample type RNA
Source name Reh cells, treatment control, 12h
Organism Homo sapiens
Characteristics cell line: Reh
tissue: leukoblasts
Treatment protocol Reh cells were seeded at a denstity of 1x10E06 cells/ml in 12 well plates with corresponding drugs added from 100x stock solutions to achieve final concentrations.
Growth protocol RPMI 1640 Medium, 10%FCS, 1%Pen/Strep, 37°C, 5%CO2
Extracted molecule total RNA
Extraction protocol mRNA was extracted by using the RNeasy Kit (Quiagen).
Label cy3
Label protocol Labeling was performed according to the protocol using the Agilent Low Input Quick Amp Labeling Kit (Agilent)
Hybridization protocol Hybridization was performed according to the Agilent 60-mer oligo microarray processing protocol using the Agilent Gene Expression Hybridization Kit (Agilent)
Scan protocol Fluorescence signals of the hybridized Agilent Microarrays were detected using Agilent’s Microarray Scanner System (Agilent).
Data processing The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent). Further data analysis was performed using the Partek® Genomics Suits software, version 6.5 beta © 2009 (Partek Inc., USA).
Submission date Oct 31, 2012
Last update date Feb 02, 2013
Contact name Shabnam Shalapour
Organization name Charité - Universitätsmedizin Berlin
Street address Augustenburger Platz 1
City Berlin
ZIP/Postal code 13353
Country Germany
Platform ID GPL13607
Series (1)
GSE41951 Gene expression profile of the human leukemia cell line Reh after treatment with bortezomib, valproic acid or a combination of both.

Data table header descriptions
VALUE Normalized signal intensity

Data table
4 2.904391
5 22.719859
6 2.064901
7 53.667313
8 52.492506
9 0.323084
10 1.198945
11 0.042089
12 36.36814
13 77.693487
14 15.56844
15 538.777235
16 0.042194
17 2.694553
18 0.042177
19 0.042155
20 16.621543
21 10.784735
24 3.26155
25 22.609904

Total number of rows: 58717

Table truncated, full table size 864 Kbytes.

Supplementary file Size Download File type/resource
GSM1028104_US22502695_1.txt.gz 12.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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