|
| Status |
Public on Jan 30, 2004 |
| Title |
Gene Expression-Based High Throughput Screening: APL Treatment with Candidate Compounds |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation. We tested several of these in duplicate replicates in blasts from a patient with APL. Also included in this data set are a collection of 6 primary patient AML cells, 3 normal neutrophils samples, and 3 normal monocyte samples. This data was used to evaluate whole genome effects of the compounds on APL cells in relation to AML versus normal neutrophils and monocytes.
Keywords = Leukemia
Keywords = APL
Keywords = AML
Keywords = chemical genomics
Keywords: repeat sample
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| |
|
| Web link |
http://www.broad.mit.edu/cancer/
|
| |
|
| Contributor(s) |
Stegmaier K, Ross KN, Colavito SA, O'Malley S, Stockwell BR, Golub TR, O’Malley S |
| Citation(s) |
14770183 |
| |
| Submission date |
Jan 16, 2004 |
| Last update date |
Aug 10, 2018 |
| Contact name |
Ken Ross |
| E-mail(s) |
kross@broadinstitute.org
|
| Phone |
617-714-7473
|
| Fax |
617-714-8955
|
| URL |
http://www.broad.mit.edu/cancer/
|
| Organization name |
Broad Institute
|
| Department |
Cancer Genomics
|
| Lab |
Golub Lab
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
|
| Samples (24)
|
|
| This SubSeries is part of SuperSeries: |
| GSE995 |
Differentiation of acute myeloid leukemia cells |
|
| Relations |
| BioProject |
PRJNA89871 |
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