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Series GSE976 Query DataSets for GSE976
Status Public on Jan 30, 2004
Title Gene Expression-Based High Throughput Screening: APL Treatment with Candidate Compounds
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We developed a general approach to small molecule library screening called GE-HTS (Gene Expression-Based High Throughput Screening) in which a gene expression signature is used as a surrogate for cellular states and applied it to the identification of compounds inducing the differentiation of acute myeloid leukemia cells. In screening 1,739 compounds, we identified 8 that reliably induced the differentiation signature, and furthermore yielded functional evidence of bona fide differentiation. We tested several of these in duplicate replicates in blasts from a patient with APL. Also included in this data set are a collection of 6 primary patient AML cells, 3 normal neutrophils samples, and 3 normal monocyte samples. This data was used to evaluate whole genome effects of the compounds on APL cells in relation to AML versus normal neutrophils and monocytes.
Keywords = Leukemia
Keywords = APL
Keywords = AML
Keywords = chemical genomics
Keywords: repeat sample
Web link
Contributor(s) Stegmaier K, Ross KN, Colavito SA, O'Malley S, Stockwell BR, Golub TR, O’Malley S
Citation(s) 14770183
Submission date Jan 16, 2004
Last update date Aug 10, 2018
Contact name Ken Ross
Phone 617-714-7473
Fax 617-714-8955
Organization name Broad Institute
Department Cancer Genomics
Lab Golub Lab
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (24)
GSM15272 APL1
GSM15394 APL2
GSM15395 APL_RA1
This SubSeries is part of SuperSeries:
GSE995 Differentiation of acute myeloid leukemia cells
BioProject PRJNA89871

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE976_RAW.tar 82.1 Mb (http)(custom) TAR (of CEL)

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