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Series GSE8839 Query DataSets for GSE8839
Status Public on Aug 22, 2007
Title Inhibition of Respiration by Nitric Oxide Induces a Mycobacterium tuberculosis Dormancy Program
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by array
Summary An estimated two billion persons are latently infected with Mycobacterium tuberculosis. The host factors that initiate and maintain this latent state and the mechanisms by which M. tuberculosis survives within latent lesions are compelling but unanswered questions. One such host factor may be nitric oxide (NO), a product of activated macrophages that exhibits antimycobacterial properties. Evidence for the possible significance of NO comes from murine models of tuberculosis showing progressive infection in animals unable to produce the inducible isoform of NO synthase and in animals treated with a NO synthase inhibitor. Here, we show that O2 and low, nontoxic concentrations of NO competitively modulate the expression of a 48-gene regulon, which is expressed in vivo and prepares bacilli for survival during long periods of in vitro dormancy. NO was found to reversibly inhibit aerobic respiration and growth. A heme-containing enzyme, possibly the terminal oxidase in the respiratory pathway, likely senses and integrates NO and O2 levels and signals the regulon. These data lead to a model postulating that, within granulomas, inhibition of respiration by NO production and O2 limitation constrains M. tuberculosis replication rates in persons with latent tuberculosis.
 
Overall design The quality controls were biological replicate and technical replicate
 
Contributor(s) Voskuil MI, Schnappinger D, Visconti KC
Citation(s) 12953092
Submission date Aug 21, 2007
Last update date Mar 17, 2012
Contact name SMD Staff
E-mail(s) array@genome.stanford.edu
Phone 650-498-6012
URL http://genome-www5.stanford.edu/
Organization name Stanford Microarray Database (SMD)
Department Stanford University, School of Medicine
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (2)
GPL8561 SMD Print_498 Mycobacterium tuberculosis
GPL9901 SMD Print_745 Mycobacterium tuberculosis
Samples (131)
GSM886248 MTB1254 Day 0 vs low Oxygen Day 4 rep 1
GSM886249 H37Rv 0 day ctrl vs Rv3132-34 KO hypoxia 4 day rep 4
GSM886250 MTB strain 1254 Ctrl vs 0.5 mM DETA/NO 40min rep 4
Relations
BioProject PRJNA102179

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE8839_RAW.tar 220.0 Kb (http)(custom) TAR
Processed data included within Sample table

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