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Series GSE66128 Query DataSets for GSE66128
Status Public on Sep 17, 2016
Title The Calcium Channel Subunit Alpha2delta2 Suppresses Axon Regeneration in the Adult CNS
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: Injuries to the adult central nervous system (CNS) often result in permanent disabilities because neurons lose the ability to regenerate their axon during development. Although the developmental transition from a growing to a transmitting phase may represent one of the first steps in the gradual loss of axon growth and regeneration ability, the molecular mechanisms regulating this process has yet to be identified. The main goal of this study was to dissect the molecular mechanisms mediating the decline in axon growth ability and its relationship with regeneration failure in the adult CNS. To this end, we sequenced the whole transcriptome of dorsal root ganglia (DRG) neurons in both growth competent and incompetent states at different developmental stages, in diverse culture and in vivo experimental conditions.
Overall design mRNA profiles of mouse embryonic E12.5 and E17.5 lumbar DRGs, conditioned and sham operated adult L4-5DRGs, and cultured adult lumbar DRG (6, 12, 24 and 36 hr after plating) neurons were generated by deep sequencing, in triplicate, using the Illumina TruSeq RNA Sample Prep Kits V2.
Contributor(s) Tedeschi A, Dupraz S, Laskowski CS, Xue J, Ulas T, Beyer M, Schultze JL, Bradke F
Citation(s) 27720483
Submission date Feb 20, 2015
Last update date May 15, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (24)
GSM1614837 06h [339]
GSM1614838 06h [340]
GSM1614839 06h [341]
BioProject PRJNA275932
SRA SRP055201

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Supplementary file Size Download File type/resource
GSE66128_Matrix_normalized.txt.gz 1.4 Mb (ftp)(http) TXT
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Processed data are available on Series record
Raw data are available in SRA

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