|
| Status |
Public on Sep 17, 2016 |
| Title |
E17.5 [337] |
| Sample type |
SRA |
| |
|
| Source name |
DRG neuron
|
| Organism |
Mus musculus |
| Characteristics |
developmental stage: embryonic 17.5
time after plating: none
|
| Growth protocol |
Adult lumbar mouse DRG were dissected, dissociated and plated at low density on laminin-coated (5 μg/ml, Roche) dishes. Neurons were cultured in Neurobasal-A medium supplemented with B-27 (Gibco), and maintained in a humidified atmosphere of 5% CO2 in air at 36.5°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were isolated by cell sorting, lyzed in Qiazol and RNA isolated. Illumina TruSeq RNA Sample Prep Kits V2 was used with 10 ng of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
Sample 23
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using TopHat with default parameters.
mRNA quantification according to RefSeq Transcripts - 2014-01-03.
raw read counts were normalized by DE-Seq.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include DE-Seq normailzed expression values for each Sample.
|
| |
|
| Submission date |
Feb 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18480 |
| Series (1) |
| GSE66128 |
The Calcium Channel Subunit Alpha2delta2 Suppresses Axon Regeneration in the Adult CNS |
|
| Relations |
| BioSample |
SAMN03356817 |
| SRA |
SRX884173 |
