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Sample GSM1614849 Query DataSets for GSM1614849
Status Public on Sep 17, 2016
Title Peripheral lesion [74]
Sample type SRA
Source name DRG neuron
Organism Mus musculus
Characteristics developmental stage: adult
time after plating: none
Growth protocol Adult lumbar mouse DRG were dissected, dissociated and plated at low density on laminin-coated (5 μg/ml, Roche) dishes. Neurons were cultured in Neurobasal-A medium supplemented with B-27 (Gibco), and maintained in a humidified atmosphere of 5% CO2 in air at 36.5°C.
Extracted molecule total RNA
Extraction protocol Cells were isolated by cell sorting, lyzed in Qiazol and RNA isolated. Illumina TruSeq RNA Sample Prep Kits V2 was used with 10 ng of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 1500
Description Sample 13
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using TopHat with default parameters.
mRNA quantification according to RefSeq Transcripts - 2014-01-03.
raw read counts were normalized by DE-Seq.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files include DE-Seq normailzed expression values for each Sample.
Submission date Feb 20, 2015
Last update date May 15, 2019
Contact name Joachim Schultze
Organization name LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
Department Genomics and Immunoregulation
Street address Carl-Troll-Strasse 31
City Bonn
State/province NRW
ZIP/Postal code 53115
Country Germany
Platform ID GPL18480
Series (1)
GSE66128 The Calcium Channel Subunit Alpha2delta2 Suppresses Axon Regeneration in the Adult CNS
BioSample SAMN03356815
SRA SRX884163

Supplementary data files not provided
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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