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| Status |
Public on Mar 01, 2015 |
| Title |
Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition (microarray) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated the response of acute myeloid cells (AML) expressing MLL-AF9 fusion gene to the pan-HDACi panobinostat (LBH589), and found that low conentrations of panobinostat lead to MLL-AF9 cell toxicity and rapid changes in gene expression.
Human hematopoietic stem/progenitor cells (HSPC) expressing MLL-AF9 fusion protein (Wei et al, Cancer Cell 2008) were cultured with 30 nM panobinostat during 6 and 24 hours. The different gene expression between cells treated and untreated was studied by gene expression analysis.
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| Overall design |
Three independent HSPC-MA9 clones were used, for each two replicates of cells after culture with 30 nM panobinostat for 6 and 24 hours and one untreated were used. A total of 16 samples were analyzed.
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| Contributor(s) |
Maiques-Diaz A, Eguileor A, Shrestha M, Gómez López G, Wunderlich M, del Rio-Machin A, Santoyo-Lopez J, Agirre X, Mulloy JC, Cigudosa JC, Alvarez S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jan 20, 2014 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Sara Alvarez |
| E-mail(s) |
salvarez@nimgenetics.com
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| Phone |
+34 672 05 98 89
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| Organization name |
NIMGenetics
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| Street address |
C/Faraday 7
|
| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL17077 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Probe Name version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE54348 |
Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition |
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| Relations |
| BioProject |
PRJNA236219 |
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