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Sample GSM1310396 Query DataSets for GSM1310396
Status Public on Mar 01, 2015
Title HSPC-MA9.31_treated_24h.rep1
Sample type RNA
Source name CD34+ cord blood cells transduced with MLL-AF9 fusion gene
Organism Homo sapiens
Characteristics cell type: CD34+ cord blood cells transduced with MLL-AF9 fusion gene
treatment: 24h exposure to 30nM panobinostat
Treatment protocol HSPC-MA9 cells were cultured with 30 nm panobinostat or vehicle. After 6 and 24 hr cells were harvested, PBS washed and pelleted.
Growth protocol Umbilical cord blood CD34+ cells were selected using immunomagnetic beads (Miltenyi Biotech) and stably transduced with retroviruses expressing empty vectors (HSPC control) or the MLL-AF9 (HSPC-MA9) fusion protein. Cells were cultured as described elsewhere ( Wei et al., cell 2008).
Extracted molecule total RNA
Extraction protocol RNA was extracted using Rneasy mini kit (Quiagen), quality was assessed using the Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol RNA was labeled using Cyanine-3 (Cy3) with low input quick amp labeling (v6.6, Sept 2012), following the manufacturer´s specificacions.
Hybridization protocol The Agilent official protocol G4140-90040 for hybridization was used
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2565CA, 3um resolution). We used the AgilentG3_GX_1color method (61x21.6 mm sscan area, Green dye channel, 3um).
Data processing The scanned images were analyzed with Feature Extraction Software v10.7 using default parameters (protocol GE1-107_Sept09) to obtain background subtracted and spatially detrended Processed Signal intensities
Submission date Jan 20, 2014
Last update date Mar 01, 2015
Contact name Sara Alvarez
Phone +34 672 05 98 89
Organization name NIMGenetics
Street address C/Faraday 7
City Madrid
State/province Madrid
ZIP/Postal code 28049
Country Spain
Platform ID GPL17077
Series (2)
GSE54218 Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition (microarray)
GSE54348 Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_23_P117082 16.19742464
A_33_P3246448 7.218742143
A_33_P3318220 6.830115484
A_33_P3236322 7.599771322
A_33_P3319925 7.74940669
A_21_P0000509 15.83167294
A_21_P0000744 15.77424994
A_24_P215804 9.473819316
A_23_P110167 16.08876611
A_33_P3211513 11.15271643
A_23_P103349 7.123407241
A_32_P61480 6.451721664
A_33_P3788124 6.603344019
A_33_P3414202 11.2530089
A_33_P3316686 12.29195659
A_33_P3300975 7.858173574
A_33_P3263061 14.82474404
A_33_P3261373 7.76374997
A_24_P278460 13.35235467
A_21_P0013109 7.182701937

Total number of rows: 50599

Table truncated, full table size 1255 Kbytes.

Supplementary file Size Download File type/resource
GSM1310396_MA9_31_24h_1.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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