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Series GSE46034 Query DataSets for GSE46034
Status Public on Dec 31, 2013
Title TAp73 is a trophic factor essential for sperm cell adhesion and maturation in testis
Organism Mus musculus
Experiment type Expression profiling by array
Summary A core evolutionary function of the p53 family is to protect the genomic integrity of gametes. However, the role of p73 in the male germline is unknown. Here we uncover that TAp73 unexpectedly functions as adhesion and maturation factor of the seminiferous epithelium orchestrating spermiogenesis. TAp73KO and p73KO mice, but not ΔNp73KO mice, display a ‘near-empty seminiferous tubule’ phenotype due to massive premature loss of immature germ cells. Its cellular basis are defective cell-cell adhesions of developing germ cells to Sertoli nurse cells, with secondary degeneration of Sertoli cells including the blood-testis-barrier, thereby disrupting the adhesive integrity and maturation of the germ epithelium. At the molecular level, TAp73, produced in germ cells, controls a coordinated transcriptional program of adhesion- and migration-related proteins including peptidase inhibitors, proteases, receptors and integrins required for germ-Sertoli cell adhesion and dynamic junctional restructuring. Thus, the testis emerges as unique organ with strict division of labor among all family members: p63 and p53 safeguard germline fidelity, while TAp73 ensures fertility by enabling sperm maturation.
 
Overall design 3 mice each for control wildtype and TAp73 knockout mice.
 
Contributor(s) Holembowski L, Kramer D, Riedel D, Dobbelstein M, Moll UM, Sordella R, Nemajerova A
Citation(s) 24662569
Submission date Apr 12, 2013
Last update date Nov 01, 2017
Contact name Gabriela Salinas
E-mail(s) Gabriela.Salinas-Riester@medizin.uni-goettingen.de
Organization name Universitaetsmedizin Goettingen
Department Department of Human Genetics
Lab NGS Integrative Genomics
Street address Justus-von-Liebig-Weg 11
City Goettingen
State/province Lower-Saxony
ZIP/Postal code 37077
Country Germany
 
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (6)
GSM1122112 TAp73KO_1
GSM1122113 TAp73KO_2
GSM1122114 TAp73KO_3
Relations
BioProject PRJNA196938

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE46034_RAW.tar 13.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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