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Sample GSM1122116 Query DataSets for GSM1122116
Status Public on Dec 31, 2013
Title WT_2
Sample type RNA
 
Source name TAp73WT testis 2
Organism Mus musculus
Characteristics strain/background: C57BL/6J
genotype/variation: TAp73 WT
age: 10 weeks
proband sex: male
tissue: testis
Treatment protocol mice were euthanized with carbon dioxide and the whole testis was removed
Growth protocol animals were housed at the mouse facility of the ENI, University Göttingen
Extracted molecule total RNA
Extraction protocol mRNA was isolated from murine testis. Tissue samples were transferred into trizol buffer, homogenized and mRNA was isolated in a single-step acid guanidinium thiocyanate-phenol-chloroform extraction following manufacturer´s instructions (http://tools.invitrogen.com/content/sfs/manuals/trizol_reagent.pdf). RNA-precipitation was done with Ethanol. RNA was diluted in nuclease-free water and concentrations were determined using a Nanodrop spectrophotometer.
Label biotin
Label protocol For each array 200 ng RNA was processed and Cy3 labeled by the Low Input Quick Amp Labeling Kit (Agilent Technologies).
 
Hybridization protocol Hybridization was performed in the hybridization oven (Agilent Technologies, 17h, 10 rpm, 65˚C)
Scan protocol Cy3 intensities were detected by a one-color microarray scanner (G2505C, Agilent Technologies) at 5 µm resolution
Description Replicate 2.
Data processing Data were quantile normalized using R and Bioconductor package limma.
 
Submission date Apr 12, 2013
Last update date Dec 31, 2013
Contact name Gabriela Salinas
E-mail(s) Gabriela.Salinas-Riester@medizin.uni-goettingen.de
Organization name Universitaetsmedizin Goettingen
Department Department of Human Genetics
Lab NGS Integrative Genomics
Street address Justus-von-Liebig-Weg 11
City Goettingen
State/province Lower-Saxony
ZIP/Postal code 37077
Country Germany
 
Platform ID GPL11202
Series (1)
GSE46034 TAp73 is a trophic factor essential for sperm cell adhesion and maturation in testis

Data table header descriptions
ID_REF
VALUE limma normalized signal intensity

Data table
ID_REF VALUE
A_51_P100034 57347.6
A_51_P100174 67107.6
A_51_P100208 6.3
A_51_P100289 2302.7
A_51_P100298 75.9
A_51_P100309 11.9
A_51_P100327 33.6
A_51_P100347 23.3
A_51_P100519 254.5
A_51_P100537 34964.4
A_51_P100573 737.4
A_51_P100624 48.1
A_51_P100625 12.9
A_51_P100768 5.8
A_51_P100776 1399.3
A_51_P100787 8893.1
A_51_P100828 4817.1
A_51_P100852 22.3
A_51_P100991 783.3
A_51_P100997 134.9

Total number of rows: 39429

Table truncated, full table size 754 Kbytes.




Supplementary file Size Download File type/resource
GSM1122116_US22502691_252665512155_S01_GE1_107_Sep09_1_2.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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