|
| Status |
Public on Dec 31, 2013 |
| Title |
WT_3 |
| Sample type |
RNA |
| |
|
| Source name |
TAp73WT testis 3
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
genotype/variation: TAp73 WT
age: 10 weeks
proband sex: male
tissue: testis
|
| Treatment protocol |
mice were euthanized with carbon dioxide and the whole testis was removed
|
| Growth protocol |
animals were housed at the mouse facility of the ENI, University Göttingen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
mRNA was isolated from murine testis. Tissue samples were transferred into trizol buffer, homogenized and mRNA was isolated in a single-step acid guanidinium thiocyanate-phenol-chloroform extraction following manufacturer´s instructions (http://tools.invitrogen.com/content/sfs/manuals/trizol_reagent.pdf). RNA-precipitation was done with Ethanol. RNA was diluted in nuclease-free water and concentrations were determined using a Nanodrop spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
For each array 200 ng RNA was processed and Cy3 labeled by the Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
Hybridization was performed in the hybridization oven (Agilent Technologies, 17h, 10 rpm, 65˚C)
|
| Scan protocol |
Cy3 intensities were detected by a one-color microarray scanner (G2505C, Agilent Technologies) at 5 µm resolution
|
| Description |
Replicate 3.
|
| Data processing |
Data were quantile normalized using R and Bioconductor package limma.
|
| |
|
| Submission date |
Apr 12, 2013 |
| Last update date |
Dec 31, 2013 |
| Contact name |
Gabriela Salinas |
| E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
| Organization name |
Universitaetsmedizin Goettingen
|
| Department |
Department of Pathology
|
| Lab |
NGS Integrative Genomics
|
| Street address |
Kreuzbergring 57
|
| City |
Goettingen |
| State/province |
Lower-Saxony |
| ZIP/Postal code |
37075 |
| Country |
Germany |
| |
|
| Platform ID |
GPL11202 |
| Series (1) |
| GSE46034 |
TAp73 is a trophic factor essential for sperm cell adhesion and maturation in testis |
|
