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Status |
Public on Dec 31, 2013 |
Title |
WT_3 |
Sample type |
RNA |
|
|
Source name |
TAp73WT testis 3
|
Organism |
Mus musculus |
Characteristics |
strain/background: C57BL/6J genotype/variation: TAp73 WT age: 10 weeks proband sex: male tissue: testis
|
Treatment protocol |
mice were euthanized with carbon dioxide and the whole testis was removed
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Growth protocol |
animals were housed at the mouse facility of the ENI, University Göttingen
|
Extracted molecule |
total RNA |
Extraction protocol |
mRNA was isolated from murine testis. Tissue samples were transferred into trizol buffer, homogenized and mRNA was isolated in a single-step acid guanidinium thiocyanate-phenol-chloroform extraction following manufacturer´s instructions (http://tools.invitrogen.com/content/sfs/manuals/trizol_reagent.pdf). RNA-precipitation was done with Ethanol. RNA was diluted in nuclease-free water and concentrations were determined using a Nanodrop spectrophotometer.
|
Label |
biotin
|
Label protocol |
For each array 200 ng RNA was processed and Cy3 labeled by the Low Input Quick Amp Labeling Kit (Agilent Technologies).
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|
|
Hybridization protocol |
Hybridization was performed in the hybridization oven (Agilent Technologies, 17h, 10 rpm, 65˚C)
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Scan protocol |
Cy3 intensities were detected by a one-color microarray scanner (G2505C, Agilent Technologies) at 5 µm resolution
|
Description |
Replicate 3.
|
Data processing |
Data were quantile normalized using R and Bioconductor package limma.
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|
|
Submission date |
Apr 12, 2013 |
Last update date |
Dec 31, 2013 |
Contact name |
Gabriela Salinas |
E-mail(s) |
Gabriela.Salinas-Riester@medizin.uni-goettingen.de
|
Organization name |
Universitaetsmedizin Goettingen
|
Department |
Department of Pathology
|
Lab |
NGS Integrative Genomics
|
Street address |
Kreuzbergring 57
|
City |
Goettingen |
State/province |
Lower-Saxony |
ZIP/Postal code |
37075 |
Country |
Germany |
|
|
Platform ID |
GPL11202 |
Series (1) |
GSE46034 |
TAp73 is a trophic factor essential for sperm cell adhesion and maturation in testis |
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