|Public on Dec 31, 2013
|TAp73WT testis 3
genotype/variation: TAp73 WT
age: 10 weeks
proband sex: male
|mice were euthanized with carbon dioxide and the whole testis was removed
|animals were housed at the mouse facility of the ENI, University Göttingen
|mRNA was isolated from murine testis. Tissue samples were transferred into trizol buffer, homogenized and mRNA was isolated in a single-step acid guanidinium thiocyanate-phenol-chloroform extraction following manufacturer´s instructions (http://tools.invitrogen.com/content/sfs/manuals/trizol_reagent.pdf). RNA-precipitation was done with Ethanol. RNA was diluted in nuclease-free water and concentrations were determined using a Nanodrop spectrophotometer.
|For each array 200 ng RNA was processed and Cy3 labeled by the Low Input Quick Amp Labeling Kit (Agilent Technologies).
|Hybridization was performed in the hybridization oven (Agilent Technologies, 17h, 10 rpm, 65˚C)
|Cy3 intensities were detected by a one-color microarray scanner (G2505C, Agilent Technologies) at 5 µm resolution
|Data were quantile normalized using R and Bioconductor package limma.
|Apr 12, 2013
|Last update date
|Dec 31, 2013
|Department of Human Genetics
|NGS Integrative Genomics
|TAp73 is a trophic factor essential for sperm cell adhesion and maturation in testis