 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 23, 2013 |
| Title |
Gene expression signatures in HuH7 cells transfected with super hybrid p53 version 2. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The p53 tumor suppressor belongs to a gene family that includes two other structurally and functionally related members: p63 and p73. However, the regulation of p53 activity differs significantly from that of p73 and p63. To enhance the tumor suppressive activity of p53, we created a p63/p53 hybrid molecule, named p63-53O, which comprises the transcriptional activation and DNA-binding domains of TAp63γ and the oligomerization domain of p53. In this study, we generated a series of p63-53O derivatives and developed an advanced hybrid molecule named “Super Hybrid p53 (SHp53).” SHp53 efficiently transactivated several proapoptotic genes, including CASP10, KCNK3, and PYCARD, compared with p53. Moreover, the silencing of these proapoptotic genes partially abolished the apoptotic response to SHp53 in human cancer cells. The potency of SHp53 for suppressing tumorigenesis was also evaluated using in vivo models. Thus, our results identify a better p63/p53 hybrid molecule for the development of anti-cancer therapies.
|
| |
|
| Overall design |
HuH-7 human hepatocellular carcinoma cells were either transduced with adenoviral vectors expressing p53, TAp63gamma, p53-p63 hybrid (super hybrid p53 version 2, p63-53O-FL), or lacZ. After 24 hours, total RNA was isolated and analyzed by hybridization to Agilent-028004 SurePrint G3 Human GE 8x60K Microarray.
|
| |
|
| Contributor(s) |
Kashima L, Sasaki Y, Tokino T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Nov 26, 2012 |
| Last update date |
Nov 27, 2018 |
| Contact name |
Yasushi Sasaki |
| E-mail(s) |
yasushi@sapmed.ac.jp
|
| Phone |
81-11-611-2111
|
| Fax |
81-11-618-3313
|
| Organization name |
Sapporo Medical University
|
| Department |
Cancer Research Institute
|
| Lab |
Molecular Biology
|
| Street address |
S-1, W-17, Chuo-ku
|
| City |
Sapporo |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA182146 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE42493_RAW.tar |
53.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...