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Series GSE42493 Query DataSets for GSE42493
Status Public on May 23, 2013
Title Gene expression signatures in HuH7 cells transfected with super hybrid p53 version 2.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The p53 tumor suppressor belongs to a gene family that includes two other structurally and functionally related members: p63 and p73. However, the regulation of p53 activity differs significantly from that of p73 and p63. To enhance the tumor suppressive activity of p53, we created a p63/p53 hybrid molecule, named p63-53O, which comprises the transcriptional activation and DNA-binding domains of TAp63γ and the oligomerization domain of p53. In this study, we generated a series of p63-53O derivatives and developed an advanced hybrid molecule named “Super Hybrid p53 (SHp53).” SHp53 efficiently transactivated several proapoptotic genes, including CASP10, KCNK3, and PYCARD, compared with p53. Moreover, the silencing of these proapoptotic genes partially abolished the apoptotic response to SHp53 in human cancer cells. The potency of SHp53 for suppressing tumorigenesis was also evaluated using in vivo models. Thus, our results identify a better p63/p53 hybrid molecule for the development of anti-cancer therapies.
Overall design HuH-7 human hepatocellular carcinoma cells were either transduced with adenoviral vectors expressing p53, TAp63gamma, p53-p63 hybrid (super hybrid p53 version 2, p63-53O-FL), or lacZ. After 24 hours, total RNA was isolated and analyzed by hybridization to Agilent-028004 SurePrint G3 Human GE 8x60K Microarray.
Contributor(s) Kashima L, Sasaki Y, Tokino T
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Submission date Nov 26, 2012
Last update date Nov 27, 2018
Contact name Yasushi Sasaki
Phone 81-11-611-2111
Fax 81-11-618-3313
Organization name Sapporo Medical University
Department Cancer Research Institute
Lab Molecular Biology
Street address S-1, W-17, Chuo-ku
City Sapporo
ZIP/Postal code 060-8556
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (4)
GSM1040914 HuH-7 lacZ-24h
GSM1040915 HuH-7 FLAF-p53-24h
GSM1040916 HuH-7 FLAG-TAp63 gamma-24h
BioProject PRJNA182146

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE42493_RAW.tar 53.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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