|
| Status |
Public on May 23, 2013 |
| Title |
HuH-7 FLAF-p53-24h |
| Sample type |
RNA |
| |
|
| Source name |
HuH-7 human hepatocellular carcinoma cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell transfection: p53 expressing adenovirus for 24 h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reagent (Invitrogen).
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was labeled using a Low Input Quick Amp Labeling Kit One-Color (Agilent Technologies) according to manufacturer's instruction.
|
| |
|
| Hybridization protocol |
Hybridization was performed using a Gene Expression Hybridization kit (Agilent Technologies) according to manufacturer's instruction.
|
| Scan protocol |
Array was scanned with an Agilent G2565BA Microarray Scanner.
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction.
|
| |
|
| Submission date |
Nov 26, 2012 |
| Last update date |
May 23, 2013 |
| Contact name |
Yasushi Sasaki |
| E-mail(s) |
yasushi@sapmed.ac.jp
|
| Phone |
81-11-611-2111
|
| Fax |
81-11-618-3313
|
| Organization name |
Sapporo Medical University
|
| Department |
Cancer Research Institute
|
| Lab |
Molecular Biology
|
| Street address |
S-1, W-17, Chuo-ku
|
| City |
Sapporo |
| ZIP/Postal code |
060-8556 |
| Country |
Japan |
| |
|
| Platform ID |
GPL13607 |
| Series (1) |
| GSE42493 |
Gene expression signatures in HuH7 cells transfected with super hybrid p53 version 2. |
|
