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| Status |
Public on Jun 04, 2018 |
| Title |
Expression from C. elegans L1 animals |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
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| Summary |
Nutrient-driven O-GlcNAcylation of key components of the transcription machinery may epigenetically modulate gene expression in metazoans. Knockouts of the O-GlcNAc cycling enzymes in C. elegans are viable and fertile, allowing a global analysis of the impact of GlcNAcylation. Here we compare gene expression in wild type and O-GlcNAc mutants (ogt-1 and oga-1) in synchronized, fed L1 animals.
Whole genome transcriptional profiling of the O-GlcNAc cycling mutants confirmed dramatic deregulation of genes in these key pathways. As predicted, the O-GlcNAc cycling mutants show phenotypically altered lifespan and susceptibility to UV stress.
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| Overall design |
L1 larvae were synchronized by starvation for two days after hatching in sterile buffer and then plated on NGM with E. coli (OP50) and allowed to feed for 3 hours at room temperature. Wild type and mutant animals were collected and total RNA isolated for gene expression analysis.
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| Contributor(s) |
Krause M, Hanover J |
| Citation(s) |
20368426, 30250452 |
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| Submission date |
Aug 24, 2012 |
| Last update date |
Jun 11, 2019 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
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| Phone |
301-496-0175
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| Organization name |
NIDDK/NIH
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| Department |
GCL
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| Lab |
Genomics Core Lab
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| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL200 |
[Celegans] Affymetrix C. elegans Genome Array |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE18132 |
Dynamic O-GlcNAc cycling at promoters of C. elegans genes regulating Longevity, Stress, and Immunity |
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| Relations |
| BioProject |
PRJNA173791 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE40371_RAW.tar |
89.2 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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