|
| Status |
Public on Jun 04, 2018 |
| Title |
Ogt Fed L1, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
ogt-1(ok430) L1 animal
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
genotype/variation: ogt mutant
development stage: L1
|
| Treatment protocol |
Synchronized L1 animals were isolated for wild type and two mutant strains: loss of the O-GlcNAc transferase ogt-1(ok430) and loss of the O-GlcNAcase oga-1(ok1207). Animals were plated on NGM with E. coli (OP50) and allowed to feed for 3 hours prior to collection and RNA isolation.
|
| Growth protocol |
Embryos were isolated from wild type and mutant gravid adults by bleach treatment and shaken gently with good aeration in sterile buffer to hatch and collected 48 hours later. Synchronized L1 animals were plated on NGM with food for 3 hours, collected, washed, and used for total RNA preparation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to standard protocols
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 4 ug total RNA
|
| |
|
| Hybridization protocol |
C. elegans whole genome expression array chips were washed and stained in the Affymetrix Fluidics Station 400s
|
| Scan protocol |
Chips were scanned with an Affymetrix Scanner with standard settings
|
| Data processing |
Data was analyzed by Micrarray Suite 5.0 (MAS5) and by RMA using the program Partek
|
| |
|
| Submission date |
Aug 24, 2012 |
| Last update date |
Jun 04, 2018 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
|
| Phone |
301-496-0175
|
| Organization name |
NIDDK/NIH
|
| Department |
GCL
|
| Lab |
Genomics Core Lab
|
| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL200 |
| Series (2) |
| GSE18132 |
Dynamic O-GlcNAc cycling at promoters of C. elegans genes regulating Longevity, Stress, and Immunity |
| GSE40371 |
Expression from C. elegans L1 animals |
|
