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| Status |
Public on Jul 10, 2012 |
| Title |
Gene Expression Response to Interferon Treatment at 12h and 24 in A549 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this experiment was to use global gene expression profiling to understand human lung epithelial cellular responses to interferon treatment.
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| Overall design |
A549 cells were treated individually with IFN-α (10 ng ml−1; Sigma I4276), IFN-β (500 U ml−1; Sigma I4151) and IFN-γ (10 ng ml−1; Sigma I32265) or mock-treated. At 12 and 24 h post-treatment, cells were harvested (n = 3 wells per condition) and total RNA was extracted and analysed by microarray. Individual well replicates were pooled for each array by combining equal masses of total RNA (n=1 array per time point for each treatment and control).
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| Contributor(s) |
Tisoncik JR, Billharz R, Burmakina S, Belisle SE, Proll SC, Korth MJ, Garcia-Sastre A, Katze MG |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 09, 2012 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
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| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Michael G. Katze, Ph.D
|
| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE39202 |
NS1 protein of influenza A virus and interferon |
|
| Relations |
| BioProject |
PRJNA170196 |
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