|
| Status |
Public on Jul 10, 2012 |
| Title |
IFN-α 24h A549 pooled |
| Sample type |
RNA |
| |
|
| Source name |
A549 Cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: IFN-alpha
time: 24hr
cell line: A549
|
| Treatment protocol |
Cells were prepared as TRIzol homogenates for subsequent RNA extraction.
|
| Growth protocol |
A549 cells were treated with IFN-alpha, IFN-beta, IFN-gamma, or mock treated. Samples were harvested for microarray analysis at 12 and 24hr post-treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
All TRIzol lysates were processed simultaneously: they were phase-separated, and RNA was isolated from the aqueous phase (diluted 2 fold with RLT buffer) using Qiagen RNeasy Mini columns and the manufacturer’s recommended protocol (Qiagen Inc., Valencia, CA). RNA quality was assessed on an Agilent 2100 Bioanalyzer using the nanochip format, and only intact RNA was used for microarray analyses.
|
| Label |
Cy3
|
| Label protocol |
The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
|
| |
|
| Hybridization protocol |
The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
|
| Scan protocol |
Dry slides were scanned on an Agilent DNA microarray scanner (Model G2505C).
|
| Data processing |
Raw images were analyzed using the Agilent Feature Extraction software (version 9.5.3.1) and the GE1-v5_95_Feb07 extraction protocol.
|
| |
|
| Submission date |
Jul 09, 2012 |
| Last update date |
Jul 10, 2012 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
|
| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Michael G. Katze, Ph.D
|
| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
| |
|
| Platform ID |
GPL4133 |
| Series (2) |
| GSE39199 |
Gene Expression Response to Interferon Treatment at 12h and 24 in A549 cells |
| GSE39202 |
NS1 protein of influenza A virus and interferon |
|
