NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM957989 Query DataSets for GSM957989
Status Public on Jul 10, 2012
Title IFN-β 24h A549 pooled
Sample type RNA
 
Source name A549 Cells
Organism Homo sapiens
Characteristics treatment: IFN-beta
time: 24hr
cell line: A549
Treatment protocol Cells were prepared as TRIzol homogenates for subsequent RNA extraction.
Growth protocol A549 cells were treated with IFN-alpha, IFN-beta, IFN-gamma, or mock treated. Samples were harvested for microarray analysis at 12 and 24hr post-treatment.
Extracted molecule total RNA
Extraction protocol All TRIzol lysates were processed simultaneously: they were phase-separated, and RNA was isolated from the aqueous phase (diluted 2 fold with RLT buffer) using Qiagen RNeasy Mini columns and the manufacturer’s recommended protocol (Qiagen Inc., Valencia, CA). RNA quality was assessed on an Agilent 2100 Bioanalyzer using the nanochip format, and only intact RNA was used for microarray analyses.
Label Cy3
Label protocol The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
 
Hybridization protocol The Agilent One-Color Microarray-Based Gene Expression Analysis Protocol was followed for all processing steps, including Cy3-cDNA probe preparation, hybridization and array washing.
Scan protocol Dry slides were scanned on an Agilent DNA microarray scanner (Model G2505C).
Data processing Raw images were analyzed using the Agilent Feature Extraction software (version 9.5.3.1) and the GE1-v5_95_Feb07 extraction protocol.
 
Submission date Jul 09, 2012
Last update date Jul 10, 2012
Contact name Michael Katze
E-mail(s) data@viromics.washington.edu
Organization name University of Washington
Department Microbiology
Lab Michael G. Katze, Ph.D
Street address Rosen Building 960 Republican St.
City Seattle
State/province WA
ZIP/Postal code 98109-4325
Country USA
 
Platform ID GPL4133
Series (2)
GSE39199 Gene Expression Response to Interferon Treatment at 12h and 24 in A549 cells
GSE39202 NS1 protein of influenza A virus and interferon

Data table header descriptions
ID_REF
VALUE gProcessedSignal

Data table
ID_REF VALUE
1 8.68E+04
2 3.11E+00
3 3.11E+00
4 3.12E+00
5 3.13E+00
6 3.14E+00
7 3.14E+00
8 3.15E+00
9 3.15E+00
10 3.16E+00
11 3.17E+00
12 6.17E+02
13 1.38E+01
14 8.03E+01
15 3.87E+01
16 1.80E+04
17 1.75E+01
18 1.83E+02
19 9.83E+04
20 2.46E+01

Total number of rows: 45015

Table truncated, full table size 648 Kbytes.




Supplementary file Size Download File type/resource
GSM957989_US93503719_251485060024_S01_GE1-v5_95_Feb07_1_1.txt.gz 8.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap