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| Status |
Public on Jun 05, 2012 |
| Title |
Replication-Timing Boundaries Facilitate Cell-type and Species-specific Regulation of a Rearranged Human Chromosome in Mouse |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome variation profiling by genome tiling array
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| Summary |
In multicellular organisms, developmental changes to replication timing occur in 400- 800 kb domains across half the genome. While clear examples of epigenetic control of replication timing have been described, a role for DNA sequence in mammalian replication timing has not been substantiated. To assess the role of DNA sequences in directing these changes, we profiled replication timing in mice carrying a genetically rearranged Human Chromosome 21 [Hsa21]. In two distinct mouse cell types, Hsa21 sequences maintained human-specific replication timing, except at points of Hsa21 rearrangement. Changes in replication timing at rearrangements extended up to 900 kb and consistently reconciled with the wild-type replication pattern at developmental boundaries of replication-timing domains. Our results demonstrate DNA sequencedriven regulation of Hsa21 replication timing during development and provide evidence that mammalian chromosomes consist of multiple independent units of replication timing regulation.
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| Overall design |
Profile comparison of fibroblast and T-cell cultures from trans-chromosomic mice and human and mouse controls.
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| Contributor(s) |
Pope BD, Chandra T |
| Citation(s) |
22736031 |
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| Submission date |
Jun 04, 2012 |
| Last update date |
Aug 23, 2016 |
| Contact name |
Benjamin Pope |
| Organization name |
Florida State University
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| Department |
Biological Science
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| Lab |
David Gilbert
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| Street address |
319 Stadium Dr
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| City |
Tallahassee |
| State/province |
FL |
| ZIP/Postal code |
32306-4295 |
| Country |
USA |
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| Platforms (3) |
| GPL11620 |
NimbleGen Mouse 385kb array [2006-07-26_MM8_WG_CGH] |
| GPL15225 |
NimbleGen Mouse CGH 3x720K Whole-Genome Tiling Array [100718_MM9_WG_CGH] |
| GPL15656 |
NimbleGen Human CGH 385K Chromosome 21 Tiling Array [2006-09-22_HG18_CHR21_FT] |
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| Samples (6)
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| GSM942928 |
Replication timing of Hsa21 in IMR90 fibroblasts, average of 2 biological replicates |
| GSM942929 |
Replication timing of Hsa21 in human CD4+ T lymphocytes, average of 2 biological replicates |
| GSM942930 |
Replication timing of Tc1-21 in Tc1 MEFs, average of 3 biological replicates |
| GSM942931 |
Replication timing of Tc1-21 in Tc1 mouse CD4+ T lymphocytes, average of 2 biological replicates |
| GSM942932 |
Replication timing of Tc1 MEFs, average of 2 biological replicates |
| GSM942933 |
Replication timing of Tc1 CD4+ T lymphocytes, average of 2 biological replicates |
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| Relations |
| BioProject |
PRJNA168029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE38472_RAW.tar |
303.9 Mb |
(http)(custom) |
TAR (of PAIR, TXT) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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