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| Status |
Public on Jan 01, 2015 |
| Title |
Global analysis of TGF-beta-regulated gene expression in MCF10Ca1h (M3) breast tumor xenografts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
TGF-betas have complex roles in tumorigenesis, with context-dependent effects that can either suppress or promote tumor progression. We have previously shown that TGF-beta has tumor suppressor activity in the MCF10Ca1h (M3) human breast cancer xenograft model. To identify potential molecular players in the tumor suppressor responses, we performed global gene expression analyses.
To determine which genes were regulated by TGF-beta in this tumor model in vivo, we performed gene expression arrays on tumors derived from xenografts of M3 cells with and without expression of a dominant negative TGF-beta receptor to block activity of endogenous TGF-beta.
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| Overall design |
Tumor xenografts of MCF10Ca1h (M3) cells that had been transduced ex vivo with pLPCX retrovirus with no insert (CON), or pLPCX expressing dominant negative type II TGF-beta receptor (dnTbRII) were harvested for gene expression analysis. Six biological replicates of each tumor type were used for a total of 12 samples
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| Contributor(s) |
Sato M, Kadota M, Tang B, Yang HH, Clifford RJ, Michalowski AM, Lee MP, Wakefield LM |
| Citation(s) |
24890385 |
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| Submission date |
Dec 08, 2011 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Howard Yang |
| E-mail(s) |
yanghow@mail.nih.gov
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| Phone |
2402765257
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| Organization name |
NCI
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| Street address |
9609 Medical Center Dr.
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| City |
Rockville |
| State/province |
MD |
| ZIP/Postal code |
20850 |
| Country |
USA |
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| Platforms (1) |
| GPL10739 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version] |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE34277 |
MCF10A-based xenograft model of breast cancer |
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| Relations |
| BioProject |
PRJNA156523 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE34276_M3tumor_gene_expression.txt.gz |
9.4 Mb |
(ftp)(http) |
TXT |
| GSE34276_RAW.tar |
46.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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