GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE34276 Query DataSets for GSE34276
Status Public on Jan 01, 2015
Title Global analysis of TGF-beta-regulated gene expression in MCF10Ca1h (M3) breast tumor xenografts
Organism Homo sapiens
Experiment type Expression profiling by array
Summary TGF-betas have complex roles in tumorigenesis, with context-dependent effects that can either suppress or promote tumor progression. We have previously shown that TGF-beta has tumor suppressor activity in the MCF10Ca1h (M3) human breast cancer xenograft model. To identify potential molecular players in the tumor suppressor responses, we performed global gene expression analyses.
To determine which genes were regulated by TGF-beta in this tumor model in vivo, we performed gene expression arrays on tumors derived from xenografts of M3 cells with and without expression of a dominant negative TGF-beta receptor to block activity of endogenous TGF-beta.
Overall design Tumor xenografts of MCF10Ca1h (M3) cells that had been transduced ex vivo with pLPCX retrovirus with no insert (CON), or pLPCX expressing dominant negative type II TGF-beta receptor (dnTbRII) were harvested for gene expression analysis. Six biological replicates of each tumor type were used for a total of 12 samples
Contributor(s) Sato M, Kadota M, Tang B, Yang HH, Clifford RJ, Michalowski AM, Lee MP, Wakefield LM
Citation(s) 24890385
Submission date Dec 08, 2011
Last update date Jan 25, 2019
Contact name Howard Yang
Phone 2402765257
Organization name NCI
Street address 9609 Medical Center Dr.
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (12)
GSM846202 M3 tumor control, biological rep1
GSM846203 M3 tumor control, biological rep2
GSM846204 M3 tumor control, biological rep3
This SubSeries is part of SuperSeries:
GSE34277 MCF10A-based xenograft model of breast cancer
BioProject PRJNA156523

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34276_M3tumor_gene_expression.txt.gz 9.4 Mb (ftp)(http) TXT
GSE34276_RAW.tar 46.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap