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Sample GSM846210 Query DataSets for GSM846210
Status Public on Jan 01, 2015
Title M3 tumor dnTbRII, biological rep3
Sample type RNA
 
Source name MCF10CA1h (M3) tumor, TGF-beta pathway blockade with dnTbRII
Organism Homo sapiens
Characteristics tissue: Breast cancer xenograft tumor
treatment: TGF-beta pathway blockade with dnTbRII
Treatment protocol refer to the summary for each study.
Growth protocol MCF10CA1h (M3) cells were infected ex vivo with pLPCX retrovirus with no insert (M3-CON) or pLPCX expressing dominant negative type II TGF-b receptor (M3-dnTbRII) . Cells were selected in DMEM/F12 medium supplemented with 5% Horse serum and 4ug/ml puromycin for 5 days. 5x10e5 cells were implanted orthotopically into female athymic NCr nu/nu mice.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified by RNeasy Mini Kit (Qiagen) according to manufacturer's instructions. RNA quality was checked on Agilent Bioanalyzer. All samples used for microarray analysis have high quality score (RIN >9).
Label Biotin
Label protocol 100 ng of RNA was reverse transcribed and amplified using Ambion WT expression kit following manufacturer’s suggested protocol. Sense strand cDNA was fragmented and labeled using Affymetrix WT terminal labeling kit.
 
Hybridization protocol Three replicates of each group were hybridized to Affymetrix mouse (human) Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs. Wash and stain were performed on Affymetrix Fluidics Station 450
Scan protocol Genechips were scanned using an Affymetrix GeneChip Scanner 3000 and data was collected using Affymetrix AGCC software.
Description RNA extracted by Rneasy
Data processing Data were analyzed with Partek Genomics Suite (version 6.5) software using the RMA normalization algorithm with default format. More details needed here?
Original, Summarized, Difference, Filename, Chip Type, tumor, Scan Date, gene expression
 
Submission date Dec 08, 2011
Last update date Jan 01, 2015
Contact name Howard Yang
E-mail(s) yanghow@mail.nih.gov
Phone 2402765257
Organization name NCI
Street address 9609 Medical Center Dr.
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
 
Platform ID GPL10739
Series (2)
GSE34276 Global analysis of TGF-beta-regulated gene expression in MCF10Ca1h (M3) breast tumor xenografts
GSE34277 MCF10A-based xenograft model of breast cancer

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
7896737 3.81239
7896739 3.11405
7896741 3.64148
7896743 5.29091
7896745 5.55606
7896747 8.55719
7896749 8.66982
7896751 2.77837
7896753 8.75103
7896755 4.96442
7896757 4.26649
7896758 4.35739
7896760 5.21426
7896762 11.1148
7896763 5.44361
7896764 5.03528
7896765 2.43176
7896766 6.84259
7896767 5.33386
7896768 9.28113

Total number of rows: 253002

Table truncated, full table size 3925 Kbytes.




Supplementary file Size Download File type/resource
GSM846210_T_M3_dnTbRII_3.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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