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Series GSE30177 Query DataSets for GSE30177
Status Public on May 02, 2012
Title Complement protein C1q modulates macrophage activation and inflammasome activity during the uptake of apoptotic cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary In this study, we developed a unique system using primary human autologous lymphocytes and HMDMs to characterize the effect of C1q on macrophage gene expression profiles during the uptake of apoptotic cells. Our results showed that C1q bound to autologous apoptotic lymphocytes (AL) significantly modulated the response of HMDMs to LPS by increasing expression of cytokines, chemokines and effector molecules associated with immunoregulation and by directly suppressing caspase-1 dependent cleavage of IL-1beta.
Overall design Human monocyte-derived macrophages (HMDMs) were incubated with early apoptotic lymphocytes (EAL), late apoptotic lymphocytes (LAL), C1q-EAL and C1q-LAL for 1h and then and stimulated with 10 ng/ml ultra-pure LPS. After 3 h of LPS stimulation, total RNA was extracted using the Illustra RNAspin Mini Isolation Kit. Gene expression profiles were studied using the Human Gene 1.0 ST array (Affymetrix). Slides were scanned using the Affymetrix GCOS software (performed by the microarray core facility at University of California, Irvine). Data processing and analysis were performed using JMP Genomics 5.0 software (SAS Institue Inc.). Significant differences in gene expression compared to unstimulated HMDMs were identified by ANOVA test using Holm multiple testing method and a false positive rate (alpha error) of 0.05
Contributor(s) Benoit ME, Clarke EV, Morgado P, Fraser DA, Tenner AJ
Citation(s) 22523386
Submission date Jun 23, 2011
Last update date Jan 25, 2019
Contact name Marie Emeline Benoit
Organization name UC Irvine
Department Molecular Biology and Biochemistry
Lab Tenner lab
Street address 2419 McGaugh Hall
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
Platforms (1)
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (18)
GSM747185 HMDM rep 1
GSM747186 HMDM rep 2
GSM747187 HMDM rep 3
BioProject PRJNA143937

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30177_RAW.tar 75.8 Mb (http)(custom) TAR (of CEL)
GSE30177_final_processed_data.txt.gz 136.9 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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