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Sample GSM747200 Query DataSets for GSM747200
Status Public on May 02, 2012
Title HMDM+C1q-LAL+LPS rep 1
Sample type RNA
 
Source name HMDM
Organism Homo sapiens
Characteristics targets: C1q-LAL
stimulus: LPS
Treatment protocol Apoptosis of lymphocytes was induced by g-irradiation (10Gy). HMDM were stimulated with 10 ng/ml ultra-pure LPS.
Growth protocol Lymphocytes were maintained for 7 days in RPMI1640, 10% FCS, 2 mM L-Glutamine and 1% penicillin/streptomycin (complete media) containing 50 U/ml rhIL-2. HMDM were generated from human peripheral monocytes, isolated by counter flow elutriation, by culture for 8 days in complete media containing 25 ng/ml rhM-CSF
Extracted molecule total RNA
Extraction protocol total RNA was extracted using the Illustra RNAspin Mini Isolation Kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol (performed by the personnel of the microarray facility at University of California Irvine)
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix Human Gene 1.0 ST Array. GeneChips were washed and stained in the GeneChip Fluidics Stations 450
Scan protocol GeneChips were scanned using the GeneChip 3000 Scanner 7G with Autoloader
Description 0410F-08_16_(HuGene-1_0-st-v1)
gene expression data from C1q-LAL+LPS-stimulated HMDM
QLAL1
Data processing The data were analyzed with JMP genomics (v5.0) using the basic expression workflow and median signal intensity as normalization method.
 
Submission date Jun 23, 2011
Last update date May 02, 2012
Contact name Marie Emeline Benoit
E-mail(s) mbenoit@uci.edu
Organization name UC Irvine
Department Molecular Biology and Biochemistry
Lab Tenner lab
Street address 2419 McGaugh Hall
City Irvine
State/province CA
ZIP/Postal code 92697
Country USA
 
Platform ID GPL10739
Series (1)
GSE30177 Complement protein C1q modulates macrophage activation and inflammasome activity during the uptake of apoptotic cells

Data table header descriptions
ID_REF
VALUE probeset normalized signal intensity

Data table
ID_REF VALUE
7892601 0.093412
7892698 -4.21446
7892756 -4.12775
7892815 -3.45051
7892916 1.402216
7892943 -4.12775
7892971 -4.03839
7893026 0.885657
7893348 2.5661
7893368 -4.21446
7893409 -0.08847
7893426 -3.55392
7893625 -3.9464
7893643 -3.13227
7893687 -3.9464
7893988 -4.21446
7894132 2.564031
7894198 -4.21446
7894480 2.215125
7894592 2.525455

Total number of rows: 253318

Table truncated, full table size 4173 Kbytes.




Supplementary file Size Download File type/resource
GSM747200.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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