|
| Status |
Public on Mar 21, 2011 |
| Title |
Expression analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
For the expression analysis of undifferentiated and differentiated tissues a meristem enriched fraction and young leaves (up to 3 mm leaf blade length) were isolated from clavata3-9 plants. Clavata3-9 mutants harbour larger shoot apical meristems than wild type plants enabling the preparation of meristematic tissue by manual dissection. Samples from both tissues were used for expression and histone H3 lysine 27 trimethylation (H3K27me3) analysis. Tissues for expression and H3K27me3 ChIP-chip analyses were simultaneously harvested.
|
| |
|
| Overall design |
Meristem enriched samples (XMe1 and XMe2) and young leaf samples (XLe1 and XLe2) of clavata3-9 plants were isolated and hybridised to Agilent 44 k arrays [G2519F, V4 (Agilent, Santa Clara)]. Two biological replicates were performed.
|
| |
|
| Contributor(s) |
Lafos M, Kroll P, Schubert D |
| Citation(s) |
21490956 |
| |
| Submission date |
Sep 30, 2010 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Daniel Schubert |
| E-mail(s) |
Daniel.Schubert@uni-duesseldorf.de
|
| Organization name |
Heinrich-Heine-University Duesseldorf
|
| Department |
Institute for Genetics
|
| Lab |
AG Schubert
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL6177 |
Agilent-015059 Arabidopsis 3 Oligo Microarray 4x44K G2519F (Feature Number version) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE24507 |
Gene expression and H3K27 tri-methylation analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants |
|
| Relations |
| BioProject |
PRJNA133487 |
Less...
More...