|
| Status |
Public on Mar 21, 2011 |
| Title |
leaf sample 1 (XLe1) |
| Sample type |
RNA |
| |
|
| Source name |
young leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: whole young leaves
|
| Treatment protocol |
For tissue comparison meristematic tissue (Me) and young leaves (Le) were isolated by manual dissection of whole seedlings.
|
| Growth protocol |
All plants were grown for 9 weeks under short day conditions (8 h light/16 h darkness) on soil.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with QIAGEN plant RNeasy kit (Qiagen, Hilden), Superscript II (Invitrogen, Carlsbad) was used for cDNA synthesis.
|
| Label |
Cy3
|
| Label protocol |
Probe labelling was performed by the Imagenes Corp. (Berlin, Germany) as described in the “One-Color Microarray-Based Gene Expression Analysis” Protocol (G4140-90040 Version 5.7, March 2008; http://www.genomics.agilent.com/GenericA.aspx?PageType=Platform&SubPageType=PlatformLiterature&PageID=289). A oligo dT / T7 RNA-Polymerase based protocol was used for labelling.
|
| |
|
| Hybridization protocol |
Array hybridisation was performed by the Imagenes Corp. (Berlin, Germany) as described in the “One-Color Microarray-Based Gene Expression Analysis” Protocol (G4140-90040 Version 5.7, March 2008; http://www.genomics.agilent.com/GenericA.aspx?PageType=Platform&SubPageType=PlatformLiterature&PageID=289).
|
| Scan protocol |
Scanning was performed by the Imagenes Corp. (Berlin, Germany) as described in the “One-Color Microarray-Based Gene Expression Analysis” Protocol (G4140-90040 Version 5.7, March 2008; http://www.genomics.agilent.com/GenericA.aspx?PageType=Platform&SubPageType=PlatformLiterature&PageID=289) with an Agilent Technologies Scanner G2505B US45103052.
|
| Description |
tissue specific gene expression
|
| Data processing |
The data is supplied as raw data as received from Imagenes Corp. (Berlin, Germany). The data was processed by the Imagenes Corp. (Berlin, Germany) as described in the “One-Color Microarray-Based Gene Expression Analysis” Protocol (G4140-90040 Version 5.7, March 2008; http://www.genomics.agilent.com/GenericA.aspx?PageType=Platform&SubPageType=PlatformLiterature&PageID=289).
|
| |
|
| Submission date |
Sep 30, 2010 |
| Last update date |
Mar 21, 2011 |
| Contact name |
Daniel Schubert |
| E-mail(s) |
Daniel.Schubert@uni-duesseldorf.de
|
| Organization name |
Heinrich-Heine-University Duesseldorf
|
| Department |
Institute for Genetics
|
| Lab |
AG Schubert
|
| Street address |
Universitaetsstr. 1
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6177 |
| Series (2) |
| GSE24464 |
Expression analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants |
| GSE24507 |
Gene expression and H3K27 tri-methylation analysis of meristematic tissue (Me) and young leaves (Le) isolated by manual dissection of clv3-9 plants |
|
