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| Status |
Public on Dec 01, 2010 |
| Title |
Microarray skeletal muscle PGC-1alpha-/- - beta f/f/Mlc1fCre mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Title: Total Skeletal Muscle PGC-1 Deficiency Uncouples Mitochondrial Derangements from Fiber Type Determination and Insulin Sensitivity
Abstract: Evidence is emerging that the PGC-1 coactivators serve a critical role in skeletal muscle metabolism, function, and disease. Mice with total PGC-1 deficiency in skeletal muscle (PGC-1α-/- βf/f/MLC-Cre mice) were generated and characterized. PGC-1α-/-βf/f/MLC-Cre mice exhibit a dramatic reduction in exercise performance compared to single PGC-1α- or PGC-1β-deficient mice and wild-type controls. The exercise phenotype of the PGC-1α-/-βf/f/MLC-Cre mice was associated with a marked diminution in muscle oxidative capacity and mitochondrial structural derangements consistent with fusion/fission and biogenic defects together with rapid depletion of muscle glycogen stores during exercise. Surprisingly, the skeletal muscle fiber type profile of the PGC-1α-/-βf/f/MLCCre mice was not significantly different than the wild-type mice. Moreover, insulin sensitivity and glucose tolerance were also not altered in the PGC-1α-/-βf/f/MLC-Cre mice. Taken together, we conclude that PGC-1 coactivators are necessary for the oxidative and mitochondrial programs of skeletal muscle but are dispensable for fundamental fiber type determination and insulin sensitivity.
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| Overall design |
RNA from PGC-1alpha-/- beta f/f/Mlc1fcre was obtained and gene expression pattern compared with PGC-1alpha -/-, PGC-1beta f/f, and PGC-1beta f/f/Mlc1fCre controls.
Results file descriptions:
1. GSE23365_skfloxAKO_PPexcl_genesup_GEO-8-16-2010:
This table contains genes that were upregulated ≥2.0 fold in gastrocnemius muscle from PGC-1alpha-/- - mice, PGC-1beta f/f/Mlc1fCre mice and PGC-1alpha-/- - beta f/f/Mlc1fCre mice. All groups are normalized to PGC-1beta f/f mice and values are expressed as mean±SEM. The column “description’ contains the gene name, and the column “ID” contains Agilent probe names.
2. GSE23365_skfloxAKO_PPexcl_genesdown_GEO-8-16-2010
This table contains genes that were downregulated ≤0.7 fold in gastrocnemius muscle from PGC-1alpha-/- - mice, PGC-1beta f/f/Mlc1fCre mice and PGC-1alpha-/- - beta f/f/Mlc1fCre mice. All groups are normalized to PGC-1beta f/f mice and values are expressed as mean±SEM. The column “description’ contains the gene name, and the column “ID” contains Agilent probe names.
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| Contributor(s) |
Zechner C, Kelly DP |
| Citation(s) |
21109195 |
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| Submission date |
Aug 02, 2010 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Christoph Zechner |
| Organization name |
Sanford-Burnham Medical Research Institute at Lake Nona
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| Lab |
PI: Daniel P. Kelly, MD
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| Street address |
6400 Sanger Road
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| City |
Orlando |
| State/province |
FL |
| ZIP/Postal code |
32827 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA131245 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE23365_RAW.tar |
74.8 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE23365_skfloxAKO_PPexcl_genesdown_GEO-8-16-2010.txt.gz |
363.5 Kb |
(ftp)(http) |
TXT |
| GSE23365_skfloxAKO_PPexcl_genesup_GEO-8-16-2010.txt.gz |
240.9 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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