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Sample GSM573312 Query DataSets for GSM573312
Status Public on Dec 01, 2010
Title bKOvsbflox3
Sample type RNA
 
Channel 1
Source name Gastrocnemius muscle PGC-1beta f/f
Organism Mus musculus
Characteristics sex: male
age: 12 week old
tissue: Gastrocnemius muscle
strain: C57BL6/J x sv129
genotype/variation: PGC-1beta f/f
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Trizol method.
Label Cy3
Label protocol 1 ug Cy3 and Cy5-labeled cRNA was mixed and fragmented according to the Agilent technologies protocol.
 
Channel 2
Source name Gastrocnemius muscle PGC-1alpha-/- beta f/f/Mlc1fCre
Organism Mus musculus
Characteristics sex: male
age: 12 week old
tissue: Gastrocnemius muscle
strain: C57BL6/J x sv129
genotype/variation: PGC-1alpha-/- beta f/f/Mlc1fCre
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the Trizol method.
Label Cy5
Label protocol 1 ug Cy3 and Cy5-labeled cRNA was mixed and fragmented according to the Agilent technologies protocol.
 
 
Hybridization protocol Samples were hybridized in 3 hybridizations per KO group, each normalized to control. Agilent platform Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F was used.
Scan protocol Arrays were scanned on an Axon Scanner to detect Cy3 and Cy5 flourescence.
Data processing GenePix Pro 6.0 acquisition and analysis software was used to visualize and analyze the 16-bit TIFF files generated by the scanner. After cluster analysis, sample 6 and 12 were excluded.
For further analysis, GenePix 4000B with global normalization was used to normalize the ratio of medians (635/532) representing KO vs control was used for probes with flag = 0 (present).
 
Submission date Aug 02, 2010
Last update date Oct 31, 2010
Contact name Christoph Zechner
Organization name Sanford-Burnham Medical Research Institute at Lake Nona
Lab PI: Daniel P. Kelly, MD
Street address 6400 Sanger Road
City Orlando
State/province FL
ZIP/Postal code 32827
Country USA
 
Platform ID GPL7202
Series (1)
GSE23365 Microarray skeletal muscle PGC-1alpha-/- - beta f/f/Mlc1fCre mice

Data table header descriptions
ID_REF
VALUE normalized ratio of medians (635/532) representing KO vs control
Flags

Data table
ID_REF VALUE Flags
A_51_P100021 -3.227 -100
A_51_P100034 0.029 0
A_51_P100052 -4.838 -100
A_51_P100063 0.087 0
A_51_P100084 0.929 0
A_51_P100099 3.87 -100
A_51_P100155 0.044 0
A_51_P100174 0.537 -100
A_51_P100181 -0.692 -100
A_51_P100218 3.227 -100
A_51_P100227 0.126 0
A_51_P100238 0.692 -100
A_51_P100246 1.18 -100
A_51_P100289 0.092 0
A_51_P100298 2.075 -100
A_51_P100309 3.803 -100
A_51_P100327 -0.808 -100
A_51_P100347 11.287 -100
A_51_P100379 -0.692 -100
A_51_P100428 -9.676 -100

Total number of rows: 40928

Table truncated, full table size 918 Kbytes.




Supplementary file Size Download File type/resource
GSM573312.gpr.gz 6.3 Mb (ftp)(http) GPR
Processed data included within Sample table

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