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Series GSE23321 Query DataSets for GSE23321
Status Public on Jul 13, 2011
Title Expression data from human naïve (TN), stem cell memory (TSCM), central memory (TCM) and effector memory (TEM) CD8+ T cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary An early-differentiated CD8+ memory T cell subset with stem cell-like properties (TSCM) can be identified within the naïve-like T cell population by the expression of CD95/Fas. Based on experiments including exon- and gene-level expression analysis, we provide evidence that this subset of antigen-specific cells represents an early precursor of conventional central (TCM) and effector (TEM) memory CD8+ T cells with enhanced self-renewal capacity and proliferative potential.
We identified 900 genes differentially expressed between major T cell subsets defined along with memory T cell commitment. Based on the analysis of these genes, CD95+ naïve T cells (TSCM) cluster closer to the CD8+ T memory compartment than to classical (CD95-) naïve T (TN) cells, and display an intermittent phenotype between classical TN and TCM cells in terms of all major T cell differentiation markers analyzed.
Overall design Three healthy human blood donors provided lymphocyte-enriched apheresis blood for this study after informed consent. From all samples, total RNA was isolated using an RNEasy Micro kit (Qiagen), processed by Ambion’s WT expression kit, fragmented and labeled with a WT Terminal Labeling Kit (Affymetrix), hybridized to WT Human Gene 1.0 ST arrays (Affymetrix) and stained on a Genechip Fluidics Station 450 (Affymetrix), all according to the respective manufacturer's instructions.

Samples represent "exon-level" and "gene-level" analyses.
Contributor(s) Gattinoni L, Lugli E, Ji Y, Pos Z, Paulos CM, Quigley M, De Almeida JR, Yu Z, Carpenito C, Wang E, Graham BS, Rosenberg SA, June CH, Marincola FM, Douek DC, Price D, Roederer M, Restifo NP
Citation(s) 21926977
Submission date Jul 29, 2010
Last update date Jan 25, 2019
Contact name Zoltan Pos
Phone +(36)12102930-56435
Organization name Semmelweis University
Department Dept. of Genetics, Cell- and Immunobiology
Street address 4 Nagyvarad ter
City Budapest
ZIP/Postal code H-1089
Country Hungary
Platforms (2)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
GPL10739 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [probe set (exon) version]
Samples (24)
GSM572410 Stem cell memory (TSCM) cells from Donor#1 [exon-level]
GSM572411 Central memory (TCM) cells from Donor#1 [exon-level]
GSM572412 Effector memory (TEM) cells from Donor#1 [exon-level]
BioProject PRJNA131239

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE23321_GSM572410_GSM572422_Donor#1_Tcd95_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572411_GSM572423_Donor#1_Tcm_(HuGene-1_0-st-v1).CEL.gz 4.2 Mb (ftp)(http) CEL
GSE23321_GSM572412_GSM572424_Donor#1_Tem_(HuGene-1_0-st-v1).CEL.gz 4.3 Mb (ftp)(http) CEL
GSE23321_GSM572413_GSM572425_Donor#1_Tn_(HuGene-1_0-st-v1).CEL.gz 4.2 Mb (ftp)(http) CEL
GSE23321_GSM572414_GSM572426_Donor#2_Tcd95_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572415_GSM572427_Donor#2_Tcm_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572416_GSM572428_Donor#2_Tem_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572417_GSM572429_Donor#2_Tn_(HuGene-1_0-st-v1).CEL.gz 4.2 Mb (ftp)(http) CEL
GSE23321_GSM572418_GSM572430_Donor#3_Tcd95_(HuGene-1_0-st-v1).CEL.gz 4.0 Mb (ftp)(http) CEL
GSE23321_GSM572419_GSM572431_Donor#3_Tcm_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572420_GSM572432_Donor#3_Tem_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
GSE23321_GSM572421_GSM572433_Donor#3_Tn_(HuGene-1_0-st-v1).CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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