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| Status |
Public on Jun 24, 2009 |
| Title |
Responsiveness of a frog cell line to the AHR ligands FICZ and TCDD |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
|
| Summary |
The aryl hydrocarbon receptor (AHR) mediates the toxic effects of environmental contaminants, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Frogs are very insensitive to the toxic effects of TCDD.
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| Overall design |
XLK-WG cells (ATCC) were grown to ~90% confluence in RPMI-1640 media supplemented with 20% FBS at 29 degrees and 5% CO2. Cells were subsequently exposed to 100 nM TCDD, FICZ, or DMSO vehicle (0.25%) for 3 hr prior to extraction of total RNA.
Study included three complete biological replicates of each treatment.
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| |
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| Contributor(s) |
Laub LB, Jones BD, Boundarant SS, Powell WH |
| Citation(s) |
19799885 |
| |
| Submission date |
Jun 17, 2009 |
| Last update date |
Sep 25, 2019 |
| Contact name |
Wade H Powell |
| E-mail(s) |
powellw@kenyon.edu
|
| Phone |
740-427-5396
|
| Fax |
740-427-5741
|
| URL |
http://biology.kenyon.edu
|
| Organization name |
Kenyon College
|
| Department |
Biology Dept.
|
| Street address |
302A College Park St.
|
| City |
Gambier |
| State/province |
OH |
| ZIP/Postal code |
43022 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA117383 |
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