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Status |
Public on Jun 24, 2009 |
Title |
Responsiveness of a frog cell line to the AHR ligands FICZ and TCDD |
Organism |
Xenopus laevis |
Experiment type |
Expression profiling by array
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Summary |
The aryl hydrocarbon receptor (AHR) mediates the toxic effects of environmental contaminants, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Frogs are very insensitive to the toxic effects of TCDD.
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Overall design |
XLK-WG cells (ATCC) were grown to ~90% confluence in RPMI-1640 media supplemented with 20% FBS at 29 degrees and 5% CO2. Cells were subsequently exposed to 100 nM TCDD, FICZ, or DMSO vehicle (0.25%) for 3 hr prior to extraction of total RNA. Study included three complete biological replicates of each treatment.
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Contributor(s) |
Laub LB, Jones BD, Boundarant SS, Powell WH |
Citation(s) |
19799885 |
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Submission date |
Jun 17, 2009 |
Last update date |
Sep 25, 2019 |
Contact name |
Wade H Powell |
E-mail(s) |
powellw@kenyon.edu
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Phone |
740-427-5396
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Fax |
740-427-5741
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URL |
http://biology.kenyon.edu
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Organization name |
Kenyon College
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Department |
Biology Dept.
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Street address |
302A College Park St.
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City |
Gambier |
State/province |
OH |
ZIP/Postal code |
43022 |
Country |
USA |
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Platforms (1) |
GPL1318 |
[Xenopus_laevis] Affymetrix Xenopus laevis Genome Array |
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Samples (9)
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Relations |
BioProject |
PRJNA117383 |