|
Status |
Public on Jun 24, 2009 |
Title |
TCDD 2 |
Sample type |
RNA |
|
|
Source name |
100 nM TCDD
|
Organism |
Xenopus laevis |
Characteristics |
cell line: XLK-WG
|
Treatment protocol |
Cells were treated with dimethylsulfoxide (DMSO) vehicle (0.25%) or 100 nM TCDD or 100 nM FICZ dissolved in DMSO (vehicle concentration also 0.25%)
|
Growth protocol |
Cells were grown to 90% confluence in 25 cm2 flasks
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using QIAshedder spin columns and RNeasy kits (Qiagen, Hilden, Germany) per the procedure supplied by the manufacturer.
|
Label |
biotin
|
Label protocol |
Probes were labeled by the University of Wisconsin Biotechnology CenterÕs Gene Expression Microarray Facility in Madison, Wisconsin using the MessageAmp II-Biotin Enhanced kit (Ambion). Input is 1 ug RNA.
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|
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Hybridization protocol |
Samples were hybridized to Xenopus laevis GeneChips (Affymetrix, Santa Clara, CA) according to manufacturer's protocol.
|
Scan protocol |
Hybridized chips were scanned using Affymetrix GC3000 7G scanning equipment.
|
Description |
TCDD 2 (biological rep)
|
Data processing |
Affymetrix GCOS software. Gene expression was determined using the PM/MM method in dChip (Build: Sept 10, 2007). Statistical analysis was completed via 2-way ANOVA with individual contrasts (p£0.01) using Statistical Analysis Software (SAS; Cary, NC)
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|
|
Submission date |
Jun 17, 2009 |
Last update date |
Jun 24, 2009 |
Contact name |
Wade H Powell |
E-mail(s) |
powellw@kenyon.edu
|
Phone |
740-427-5396
|
Fax |
740-427-5741
|
URL |
http://biology.kenyon.edu
|
Organization name |
Kenyon College
|
Department |
Biology Dept.
|
Street address |
302A College Park St.
|
City |
Gambier |
State/province |
OH |
ZIP/Postal code |
43022 |
Country |
USA |
|
|
Platform ID |
GPL1318 |
Series (1) |
GSE16670 |
Responsiveness of a frog cell line to the AHR ligands FICZ and TCDD |
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