|
| Status |
Public on Jun 24, 2009 |
| Title |
FICZ 3 |
| Sample type |
RNA |
| |
|
| Source name |
100 nM FICZ
|
| Organism |
Xenopus laevis |
| Characteristics |
cell line: XLK-WG
|
| Treatment protocol |
Cells were treated with dimethylsulfoxide (DMSO) vehicle (0.25%) or 100 nM TCDD or 100 nM FICZ dissolved in DMSO (vehicle concentration also 0.25%)
|
| Growth protocol |
Cells were grown to 90% confluence in 25 cm2 flasks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using QIAshedder spin columns and RNeasy kits (Qiagen, Hilden, Germany) per the procedure supplied by the manufacturer.
|
| Label |
biotin
|
| Label protocol |
Probes were labeled by the University of Wisconsin Biotechnology CenterÕs Gene Expression Microarray Facility in Madison, Wisconsin using the MessageAmp II-Biotin Enhanced kit (Ambion). Input is 1 ug RNA.
|
| |
|
| Hybridization protocol |
Samples were hybridized to Xenopus laevis GeneChips (Affymetrix, Santa Clara, CA) according to manufacturer's protocol.
|
| Scan protocol |
Hybridized chips were scanned using Affymetrix GC3000 7G scanning equipment.
|
| Description |
FICZ 5 (biological rep)
|
| Data processing |
Affymetrix GCOS software. Gene expression was determined using the PM/MM method in dChip (Build: Sept 10, 2007). Statistical analysis was completed via 2-way ANOVA with individual contrasts (p£0.01) using Statistical Analysis Software (SAS; Cary, NC)
|
| |
|
| Submission date |
Jun 17, 2009 |
| Last update date |
Jun 24, 2009 |
| Contact name |
Wade H Powell |
| E-mail(s) |
powellw@kenyon.edu
|
| Phone |
740-427-5396
|
| Fax |
740-427-5741
|
| URL |
http://biology.kenyon.edu
|
| Organization name |
Kenyon College
|
| Department |
Biology Dept.
|
| Street address |
302A College Park St.
|
| City |
Gambier |
| State/province |
OH |
| ZIP/Postal code |
43022 |
| Country |
USA |
| |
|
| Platform ID |
GPL1318 |
| Series (1) |
| GSE16670 |
Responsiveness of a frog cell line to the AHR ligands FICZ and TCDD |
|
