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| Status |
Public on Oct 27, 2021 |
| Title |
Gene expression profiling with hypoxia and BRD-K34222889 from primary human pulmonary artery endothelial cells (HPAECs) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
BRD-K34222889 is a structural analog of the anti-inflammatory and senolytic drug piperlongumine. Piperlongumine inhibits GSTP1, an enzyme that places glutathione moieties on cysteine residues of various protein targets and for modulation of protein stability. The objective of this experiment was to determine the effect of a Piperlongumine analog BRD-K34222889 in endothelial dysfunction. Interestingly, BRD-K34222889 was found to inhibit glutathione S-transferase P (GSTP1), which increased ISCU protein stability via preventing glutathionylation and thus increased oxidative metabolism and decreased PAEC apoptosis. Activites of BRD-K34222889 reversed gene transcripts altered in PAECs with hypoxia that more than half of these genes were categorized in pathways relevant to cell cycle, cell death, and metabolism and all known to be controlled by ISCU.
We use Affymetrix Clairom S platform to determine the whole gene expression.
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| Overall design |
HPAECs were induced with hypoxia with or without BRD-K34222889 and appropriate control for 48h. There were three biological replicate for each group for a total of 9 samples. The groups were Normoxia with vehicle, hypoxia with vehicle, hypoxia with BRD-K34222889
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| Contributor(s) |
Chan SY, Yang J, Speyer G, Handen AL |
| Citation(s) |
34669463 |
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| Submission date |
Oct 27, 2020 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Adam Handen |
| E-mail(s) |
adam.handen@gmail.com
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| Organization name |
University of Pittsburgh
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| Department |
Vascular Medicine Institute
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| Lab |
Chan Lab
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| Street address |
200 Lothrop Street
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| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15261 |
| Country |
USA |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA672710 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160255_RAW.tar |
11.6 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| GSE160255_matrix_AVG_STDDEV.xlsx |
3.2 Mb |
(ftp)(http) |
XLSX |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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