|
| Status |
Public on Oct 27, 2021 |
| Title |
HPAECs, Hypoxia-3 |
| Sample type |
RNA |
| |
|
| Source name |
primary pulmonary artery endothelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: primary pulmonary artery endothelial cells
stress: hypoxia
treatment: control
|
| Treatment protocol |
PAECs were treated with with vehicle control (DMSO, Sigma-41639), BRD2889 (1uM) (Broad Institute), as indicated. After exposure to hypoxia for 24 h, the cells were harvested for assay.
|
| Growth protocol |
Pulmonary arterial endothelial cells (PAECs) plated in 6-well cell culture plates at 1X 10^5 cells per well, allowed to grow for 24 h, and the media were then changed to standard media and then the cells were placed into the hypoxia chamber.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy kit (Qiagen) using extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA samples were labeled using the Affymetrix WTPLus kit according to manufacturer’s guidelines.
|
| |
|
| Hybridization protocol |
RNA samples were probed using the Clariom S Human Array.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G System.
|
| Description |
HPAECs under hypoxia and treated with vehicle.
|
| Data processing |
The gene expression was normalized with Robust Multiarray Analysis (RMA). The differential expression of the genes was determined using Affymetrix transcriptome analysis console (TAC) software.
|
| |
|
| Submission date |
Oct 27, 2020 |
| Last update date |
Oct 27, 2021 |
| Contact name |
Adam Handen |
| E-mail(s) |
adam.handen@gmail.com
|
| Organization name |
University of Pittsburgh
|
| Department |
Vascular Medicine Institute
|
| Lab |
Chan Lab
|
| Street address |
200 Lothrop Street
|
| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE160255 |
Gene expression profiling with hypoxia and BRD-K34222889 from primary human pulmonary artery endothelial cells (HPAECs) |
|
