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Series GSE145279 Query DataSets for GSE145279
Status Public on May 28, 2020
Title Single-cell RNA-seq: INCB059872 in wild-type murine bone marrow
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary INCB059872 is a selective irreversible inhibitor of Lysine-Specific Demethylase 1 (LSD1) that is in phase 1 clinical trials in hematopoietic malignancies. Mice treated with INCB059872 had reduced platelet counts within 4 days of treatment. Here, we used single-cell RNA-seq to study the effects of INCB059872 on hematopoietic progenitor populations within wild-type murine bone marrow. Our results showed that INCB059872 triggered accumulation of megakaryocyte early progenitor cells with gene expression hallmarks of stem cells, which may begin to explain the thrombocytopenia observed in patients treated with LSD1 inhibitors.
Overall design Single-cell RNA-seq of lineage-negative bone marrow from mice treated with LSD1i for 0, 4, or 6 days (3 mice per group)
Contributor(s) Johnston G, Hiebert S
Citation(s) 32422235
Submission date Feb 13, 2020
Last update date May 30, 2020
Contact name Scott Hiebert
Organization name Vanderbilt University
Street address 2220 Pierce Ave.
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (9)
GSM4314099 single cells_Control A
GSM4314100 single cells_Control B
GSM4314101 single cells_Control C
BioProject PRJNA606589
SRA SRP249376

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Supplementary file Size Download File type/resource
GSE145279_RAW.tar 112.0 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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