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Sample GSM4314107 Query DataSets for GSM4314107
Status Public on May 28, 2020
Title single cells_INCB059872 6d C
Sample type SRA
Source name lineage-negative bone marrow
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: lineage-negative bone marrow
time (days): 6
Treatment protocol Mice were treated daily with 10 mg/kg INCB059872 via oral gavage.
Extracted molecule total RNA
Extraction protocol Bone marrow was harvested from femurs and tibias and stained with BD Pharmingen Biotin Mouse Lineage Panel and streptavidin-PacBlue. Zombie-NIR Fixable Viability Dye was added to each sample at 1:1000 dilution before sorting alive lineage-negative population (PacBlue-/NIR-). Sorted cells were submitted for single-cell RNA-seq using 10X Genomics Chromium Controller.
Barcoded cDNA libraries were prepared according to instructions from 10X Genomics.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing Samples were demultiplexed and single-cell gene expression matrices were generated using Cell Ranger software.
Seurat R package was used for downstream analysis.
Genome_build: mm10
Supplementary_files_format_and_content: "filtered_feature_bc_matrix" folder contents from Cell Ranger output (Genes.tsv, Barcodes.tsv, Matrix.mtx)
Submission date Feb 13, 2020
Last update date May 28, 2020
Contact name Scott Hiebert
Organization name Vanderbilt University
Street address 2220 Pierce Ave.
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platform ID GPL24247
Series (1)
GSE145279 Single-cell RNA-seq: INCB059872 in wild-type murine bone marrow
BioSample SAMN14101641
SRA SRX7719990

Supplementary file Size Download File type/resource
GSM4314107_1433-7.tar.gz 13.1 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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