|
| Status |
Public on May 28, 2020 |
| Title |
single cells_INCB059872 4d A |
| Sample type |
SRA |
| |
|
| Source name |
lineage-negative bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: lineage-negative bone marrow
time (days): 4
|
| Treatment protocol |
Mice were treated daily with 10 mg/kg INCB059872 via oral gavage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bone marrow was harvested from femurs and tibias and stained with BD Pharmingen Biotin Mouse Lineage Panel and streptavidin-PacBlue. Zombie-NIR Fixable Viability Dye was added to each sample at 1:1000 dilution before sorting alive lineage-negative population (PacBlue-/NIR-). Sorted cells were submitted for single-cell RNA-seq using 10X Genomics Chromium Controller.
Barcoded cDNA libraries were prepared according to instructions from 10X Genomics.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Samples were demultiplexed and single-cell gene expression matrices were generated using Cell Ranger software.
Seurat R package was used for downstream analysis.
Genome_build: mm10
Supplementary_files_format_and_content: "filtered_feature_bc_matrix" folder contents from Cell Ranger output (Genes.tsv, Barcodes.tsv, Matrix.mtx)
|
| |
|
| Submission date |
Feb 13, 2020 |
| Last update date |
May 28, 2020 |
| Contact name |
Scott Hiebert |
| Organization name |
Vanderbilt University
|
| Street address |
2220 Pierce Ave.
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE145279 |
Single-cell RNA-seq: INCB059872 in wild-type murine bone marrow |
|
| Relations |
| BioSample |
SAMN14101646 |
| SRA |
SRX7719985 |
