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| Status |
Public on Dec 10, 2019 |
| Title |
Transcriptomes of human trained macrophages before and after Mtb exposure |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Trained Immunity (TRIM) is the phenomenon whereby innate immune cells such as monocytes or macrophages undergo functional reprogramming after exposure to specific microbial or metabolic components. In this study, monocytes were trained with beta-glucan and then re-exposed to Mycobacterium tuberculosis.
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| Overall design |
In this study human monocytes were exposed to beta-glucan for 24 hours in vitro, a stimulus that induced TRIM, and were then differentiated to macrophages for another 5 days. At day 6, trained and not-trained macrophages were exposed to Mycobacterium tuberculosis (Mtb) for 4 hours and their transcriptomes were profiled .
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| |
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| Contributor(s) |
Moorlag S, Novakovic B, Netea M |
| Citation(s) |
32433977 |
| |
| Submission date |
Dec 09, 2019 |
| Last update date |
Mar 20, 2023 |
| Contact name |
Boris Novakovic |
| E-mail(s) |
boris.novakovic@mcri.edu.au
|
| Phone |
0434980073
|
| Organization name |
Murdoch Children's Research Institute
|
| Street address |
Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
|
| City |
Parkville |
| State/province |
Victoria |
| ZIP/Postal code |
3052 |
| Country |
Australia |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA594316 |
| SRA |
SRP235239 |
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