GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM4210636 Query DataSets for GSM4210636
Status Public on Dec 10, 2019
Sample type SRA
Source name macrophage_6d_media
Organism Homo sapiens
Characteristics donor: Donor_A
tissue: Adult Human Peripheral Blood
cell type: macrophage
treatment protocol: 6_day_media
Treatment protocol Cells were collected in Trizol.
Growth protocol Monocytes were negatively selected from peripheral blood and cultured in RPMI and 10% human serum for 6 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNEasy mini kit.
Libraries were prepared by Novogene
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing MMSEQ package was used to infer gene expression levels. Reads were mapped to the genome using Bowtie1
Genome_build: hg19
Supplementary_files_format_and_content: RNA files are in a .gene.mmseq.txt format, containing counts per gene and log RPKM.
Submission date Dec 09, 2019
Last update date Mar 20, 2023
Contact name Boris Novakovic
Phone 0434980073
Organization name Murdoch Children's Research Institute
Street address Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
Platform ID GPL24676
Series (1)
GSE141656 Transcriptomes of human trained macrophages before and after Mtb exposure
BioSample SAMN13512250
SRA SRX7290748

Supplementary file Size Download File type/resource
GSM4210636_A_RPMI_RPMI.gene.mmseq.txt.gz 2.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap