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Sample GSM4210637 Query DataSets for GSM4210637
Status Public on Dec 10, 2019
Title B_BG_MTB
Sample type SRA
 
Source name macrophage_BG_24hr_5d_media_MTB_4h
Organism Homo sapiens
Characteristics donor: Donor_B
tissue: Adult Human Peripheral Blood
cell type: macrophage
treatment protocol: BG_24hr_5_day_media_MTB_4hr
Treatment protocol Cells were collected in Trizol.
Growth protocol Monocytes were negatively selected from peripheral blood and cultured in RPMI and 10% human serum for 6 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted using the RNEasy mini kit.
Libraries were prepared by Novogene
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing MMSEQ package was used to infer gene expression levels. Reads were mapped to the genome using Bowtie1
Genome_build: hg19
Supplementary_files_format_and_content: RNA files are in a .gene.mmseq.txt format, containing counts per gene and log RPKM.
 
Submission date Dec 09, 2019
Last update date Mar 20, 2023
Contact name Boris Novakovic
E-mail(s) boris.novakovic@mcri.edu.au
Phone 0434980073
Organization name Murdoch Children's Research Institute
Street address Murdoch Children's Research Institute, Royal Children's Hospital, Flemington Road
City Parkville
State/province Victoria
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL24676
Series (1)
GSE141656 Transcriptomes of human trained macrophages before and after Mtb exposure
Relations
BioSample SAMN13512249
SRA SRX7290749

Supplementary file Size Download File type/resource
GSM4210637_B_BG_MTB.gene.mmseq.txt.gz 2.4 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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